Development of cardiomyopathy-specific iPS cell-derived 3-dimensional biomaterial-based cardiac tissues suitable for drug discovery
| Project/Area Number |
16K10626
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Institute of Physical and Chemical Research (2017-2018)
Kyoto University (2016) |
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Principal Investigator |
Masumoto Hidetoshi 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (70645754)
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| Co-Investigator(Kenkyū-buntansha) |
池田 義 京都大学, 医学研究科, 准教授 (40281092)
南方 謙二 京都大学, 医学研究科, 講師 (60539675)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 心臓再生医療 / iPS細胞 / バイオエンジニアリング / 心筋疾患外科学 / 拡張型心筋症 / 再生医学 / 創薬研究 / バイオマテリアル |
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| Outline of Final Research Achievements |
First, we have established a protocol for cardiac cell differentiation from cardiomyopathy-specific iPS cells which is essential for the cell preparation of cardiomyopathy-specific iPS cell-derived 3-dimensional biomaterial-based cardiac tissues. Next, we constructed various types of 3-dimensional cardiac tissues and analysed them which resulted in immature tissue property and function including ion channel expression. We loaded the tissues with electrical stimulation training culture which promoted the tissue maturation which was confirmed by the expression level of mRNAs relating to tissue maturation. Here, we established a technological basis for the development of cardiomyopathy-specific iPS cell-derived 3-dimensional biomaterial-based cardiac tissues suitable for drug discovery.
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| Academic Significance and Societal Importance of the Research Achievements |
本邦においては、今後心不全患者の増加が予想されている一方で、心臓移植や植込み型人工心臓は未だ十分な供給がなされない状況がある。本研究成果は、心不全患者に対する創薬研究における、iPS細胞を用いた新たな技術基盤を与えるものであり、ヘルスケアおよび医療経済に対し貢献しうるものである。また、心筋症患者iPS細胞由来成熟化人工心臓組織は、疾患の病態をin vitroで再現しうるものであり、疾患進行に関してこれまで動物実験などで得られなかった新たな学術的発見をもたらすのに貢献するものである。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Chronic optical pacing conditioning of h-iPSC engineered cardiac tissues.2019
Author(s)
Dwenger M, Kowalski WJ, Ye F, Yuan F, Tinney JP, Setozaki S, Nakane T, Masumoto H, Campbell P, Guido W, Keller BB.
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Journal Title
J Tissue Eng
Volume: 10
Pages: 204173141984174-204173141984174
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A therapeutic angiogenesis of sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel sheets in a canine chronic myocardial infarction model.2018
Author(s)
Kumagai M, Minakata K, Masumoto H, Yamamoto M, Yonezawa A, Ikeda T, Uehara K, Yamazaki K, Ikeda T, Matsubara K, Yokode M, Shimizu A, Tabata Y, Sakata R, Minatoya K.
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Journal Title
Heart Vessels
Volume: 33
Issue: 10
Pages: 1251-1257
DOI
Related Report
Peer Reviewed
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[Presentation] Therapeutic potential of human iPS cell-derived engineered cardiac tissue including cardiomyocytes and multiple vascular lineages for myocardial infarction.2016
Author(s)
Masumoto H, Nakane T, J P.Tinney, F Yuan, F Ye, W J.Kowalski, Minakata K, Sakata R, Yamashita J K, B B.Keller.
Organizer
International Society for Stem Cell Research(ISSCR) 2016
Place of Presentation
San Francisco, USA
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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