The establishment of molecular testing using cytological materials for lung cancer treatments

• Project/Area Number: 16K10690
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Kyorin University
• Principal Investigator: Tanaka Ryota 杏林大学, 医学部, 准教授 (40415063)
• Research Collaborator: Tachibana Keisei ; Kamma Hiroshi ; Fujiwara Masachika ; Kondo Haruhiko ; Ohtsuka Kouki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 肺癌 / コンパニオン診断 / 気管支鏡 / 液状化検体細胞診 / EGFR / KRAS / ROS-1 / ALK / 肺腺癌 / 細胞診 / Liquid Based Cytology / 上皮成長因子受容体 / 液状化細胞診 / EML4-ALK融合遺伝子 / 肺外科

Outline of Final Research Achievements

We studied patients with lung cancer undergoing bronchoscopic biopsy to assist us with making treatment plans. After getting cytological samples using instrument washings, the specimen was fixed by BD CytoRich Red Preservative, making a cellblock with iPGell. The cellblocks with iPGell were used for cytomorphological subtyping, molecular testing and immunocytochemical analysis for validation of feasibility in this study. The cellblock with iPGell was suitable for use in these kinds of examinations, and it might be an optimal preparation for analyzing gene aberrations before developing treatment plans in our clinical practices.

Academic Significance and Societal Importance of the Research Achievements

非小細胞肺癌の薬物療法は分子標的治療や、免疫療法がめざましい発展を遂げてきた。気管支鏡下生検による確定診断は重要であり、薬剤耐性後の再生検も更に必要なことがある。本研究において微量検体を用いた免疫組織学的な組織型推定、更に分子標的治療薬などの効果予測となる遺伝子解析を高感度に実行した。そして細胞浮遊液ゼリー化試薬（iPGell）を用いた方法の検証実験を行い、本法が簡便かつ効率的な方法であることを証明した。

Research Products

• [Journal Article] Genotyping and cytomorphological subtyping of lung adenocarcinoma based on liquid-based cytology (2019)
  • Author(s): Tanaka Ryota、Sakamoto Norihiko、Suzuki Hitomi、Tachibana Keisei、Ohtsuka Kouki、Kishimoto Koji、Fujiwara Masachika、Kamma Hiroshi、Shibahara Junji、Kondo Haruhiko
  • Journal Title: Diagnostic Cytopathology Volume: 印刷中 Issue: 6 Pages: 564-570
  • DOI: 10.1002/dc.24154
  • Peer Reviewed / Open Access
• [Journal Article] Video review program enhances resident training in laparoscopic inguinal hernia: a randomized blinded controlled trial (2018)
  • Author(s): Tanaka Ryota、DeAsis Francis、Vigneswaran Yalini、Linn John、Carbray JoAnn、Denham Woody、Haggerty Stephen、Ujiki Michael
  • Journal Title: Surgical Endoscopy Volume: 32 Issue: 6 Pages: 2847-2851
  • DOI: 10.1007/s00464-017-5992-0
  • Peer Reviewed / Open Access
• [Journal Article] A severe combined immunodeficiency disease mouse model of human adenocarcinoma with lepidic-predominant growth (2018)
  • Author(s): Tanaka Ryota、Tachibana Keisei、Suda Kazuharu、Kondo Haruhiko、Noguchi Masayuki
  • Journal Title: Pathology - Research and Practice Volume: 214 Issue: 12 Pages: 2000-2003
  • DOI: 10.1016/j.prp.2018.09.021
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Reprimo Down-regulation on Malignant Transformation of Intraductal Papillary Mucinous Neoplasm (2018)
  • Author(s): Nakazato Tetsuya、Suzuki Yutaka、Tanaka Ryota、Abe Nobutsugu、Masaki Tadahiko、Mori Toshiyuki、Ohkura Yasuo、Sugiyama Masanori
  • Journal Title: Pancreas Volume: 47 Issue: 3 Pages: 291-295
  • DOI: 10.1097/mpa.0000000000001002
  • Peer Reviewed
• [Journal Article] The Cytomorphological Characteristics of Non-small Cell Lung Cancer are Associated with Its Radiological Features (2017)
  • Author(s): Tanaka Ryota、Sakamoto Norihiko、Suzuki Hitomi、Tachibana Keisei、Takei Hidefumi、Kishimoto Koji、Fujiwara Masachika、Kamma Hiroshi、Shibahara Junji、Kondo Haruhiko
  • Journal Title: Journal of Cytology & Histology Volume: 08 Issue: 05 Pages: 482-482
  • DOI: 10.4172/2157-7099.1000482
  • Peer Reviewed
• [Journal Article] A Comparative Assessment of Novel Mini-Laparoscopic Tools (2017)
  • Author(s): Dorian Emily D.、DeAsis Francis J.、Lapin Brittany、Amesbury Robert、Tanaka Ryota、Ujiki Michael B.
  • Journal Title: Surgical Innovation Volume: 24 Issue: 1 Pages: 35-41
  • DOI: 10.1177/1553350616667351
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 呼吸器細胞診における細胞採取と検体処理法 (2018)
  • Author(s): 田中良太、坂本憲彦、鈴木 瞳、新井信晃、橘 啓盛、岸本浩次、藤原正親、柴原純二、菅間 博、近藤晴彦
  • Organizer: 第57回日本臨床細胞学会秋期大会
• [Presentation] Comparison between Cytomorphological Characteristics and Radiological Features in Non-small Cell Lung Cancer (2018)
  • Author(s): 1.Tanaka R, Sakamoto N, Suzuki H, Tachibana K, Takei H, Kishimoto K, Fujiwara M, Kamma H, Shibahara J, Kondo H
  • Organizer: The 17th Korea-Japan Joint Meeting for Diagnostic Cytopathology
  • Int'l Joint Research
• [Presentation] The value of repeated metastasectomy (2018)
  • Author(s): Tanaka R
  • Organizer: Fourth International Joint Meeting on Thoracic Surgery
  • Int'l Joint Research / Invited
• [Presentation] The especial case of interstitial lung disease (2018)
  • Author(s): Tanaka R
  • Organizer: Fourth International Joint Meeting on Thoracic Surgery
  • Int'l Joint Research / Invited
• [Presentation] 肺癌の細胞診と画像診断―細胞形態と画像所見との対比― (2017)
  • Author(s): 田中良太、坂本憲彦、鈴木 瞳、橘 啓盛、中里陽子、武井秀史、藤原正親、柴原純二、菅間 博、近藤晴彦
  • Organizer: 第58回日本臨床細胞学会総会（春期大会）
• [Presentation] 肺癌診療領域におけるLBCを用いた遺伝子プロファイリング (2017)
  • Author(s): 田中良太、坂本憲彦、鈴木 瞳、中里陽子、橘 啓盛、武井秀史、大塚弘毅、岸本浩次、藤原正親、柴原純二、菅間 博、近藤晴彦
  • Organizer: 第56回日本臨床細胞学会秋期大会
• [Presentation] Differentiating of Cytomorphological Characteristics in Non-small Cell Lung Cancer Predicts Value of Radiologic Features (2017)
  • Author(s): Tanaka R, Fujiwara M, Tachibana K, Miura J, Shimizu R, Nagashima Y, Miya T, Takei H, Shibahara J, Kamma H, Kondo H
  • Organizer: IASLC WCLC 2017
  • Int'l Joint Research
• [Presentation] 肺癌個別化治療における病理・細胞診断 (2016)
  • Author(s): 田中良太
  • Organizer: 第30回関東臨床細胞学会学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2016-09-10
  • Invited
• [Presentation] The Efficiency of Histological Subtyping and Molecular Testing in Bronchoscopic Examinations for Lung Cancer Diagnosis (2016)
  • Author(s): Tanaka R, Tachibana K, Sakamoto N, Fujiwara M, Kanma H, Kondo H
  • Organizer: The 19th International Congress of Cytology
  • Place of Presentation: Yokohama
  • Year and Date: 2016-05-29
  • Int'l Joint Research