Glial metabolism evaluated by acetate positron emission computed tomography in chronic progressive brain ischemia model in rats

• Project/Area Number: 16K10713
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: University of Fukui
• Principal Investigator: Kikuta Kenichiro 福井大学, 学術研究院医学系部門, 教授 (90332725)
• Research Collaborator: Okazawa Hidehiko
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ラット / 進行性脳虚血 / モデル / アミロ イドコンストリクター / 全脳虚血 / 脳虚血 / タウ蛋白 / 慢性脳虚血モデル / 血行力学的 / 内頸動脈狭窄症 / アミロイドコンストリクター

Outline of Final Research Achievements

Under general anesthesia by inhalation 1.5% isoflrene, bilateral vertebral arteries of 10-12 weeks old of Splague-Douley rats were coagulated and cut by bipolar coagualators. 2-3 days after, second operation was performed. Under general anesthesia, bilateral common, external, and internal carotid arteries were surgically exposed. Amyloid constrictor invented by Inohara et al was attached to right common carotid artery and contralateral internal carotid artery was ligated. Progressive hole brain ischemia could be obtained by this model but surgical mortality amounted to around 80%.

Academic Significance and Societal Importance of the Research Achievements

全脳モデルが作成できればもやもや病など進行性脳血管障害の治療に寄与すると考えられたが、本研究はモデルの致死率が高く、両側ではなく片側頸動脈の操作にモデルを変更して検討している。

Research Products

• [Journal Article] Noninvasive method for measurement of cerebral blood flow using O-15 water PET/MRI with ASL correlation. (2018)
  • Author(s): Okazawa H, Higashino Y, Tetsuya Tsujikawa T, Arishima H, Mori T, Kiyono Y, Kimura H, Kikuta K.
  • Journal Title: Eur J Radiol Volume: 105 Pages: 102-109
  • DOI: 10.1016/j.ejrad.2018.05.033
  • Peer Reviewed
• [Journal Article] Pathological examination of cerebral amyloid angiopathy in patients who underwent removal of lobar hemorrhages. (2018)
  • Author(s): Lin CM, Arishima H, Kikuta KI, Naiki H, Kitai R, Kodera T, Matsuda K, Hashimoto N, Isozaki M, Tsunetoshi K, Neishi H, Higashino Y, Akazawa A, Arai H, Yamada S
  • Journal Title: Journal of Neurology Volume: 265 Issue: 3 Pages: 567-577
  • DOI: 10.1007/s00415-018-8740-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Precise Evaluation of Striatal Oxidative Stress Corrected for Severity of Dopaminergic Neuronal Degeneration in Patients with Parkinson’s Disease: A Study with ⁶²Cu-ATSM PET and ¹²³I-FP-CIT SPECT (2017)
  • Author(s): Neishi H, Ikawa M, Okazawa H, Tsujikawa T, Arishima H, Kikuta KI, Yoneda M
  • Journal Title: European Neurology Volume: 78 Issue: 3-4 Pages: 161-168
  • DOI: 10.1159/000479627
  • Peer Reviewed / Open Access
• [Journal Article] Arterial Transit Time Mapping Obtained by Pulsed Continuous 3D ASL Imaging with Multiple Post-Label Delay Acquisitions: Comparative Study with PET-CBF in Patients with Chronic Occlusive Cerebrovascular Disease (2016)
  • Author(s): T. Tsujikawa, H. Kimura, T. Matsuda, Y. Fujiwara, M. Isozaki, K. Kikuta, H. Okazawa
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: 1-21
  • DOI: 10.1371/journal.pone.0156005
  • Peer Reviewed / Open Access
• [Journal Article] Long-term follow-up of cryopreservation with glycerol of autologous bone flaps for cranioplasty after decompressive craniectomy (2016)
  • Author(s): Takeuchi H, Higashino Y, Hosoda T, Yamada S, Arishima H, Kodera T, Kitai R, Kikuta K
  • Journal Title: Acta Neurochir (Wien) Volume: 58 Issue: 3 Pages: 571-575
  • DOI: 10.1007/s00701-015-2679-y
  • Peer Reviewed
• [Journal Article] Clinicopathologic features of small cell glioblastomas (2016)
  • Author(s): Takeuchi H, Kitai R, Hosoda T, Yamada S, Hashimoto N, Kikuta K, Shimizu Y, Kimura H
  • Journal Title: J Neurooncol Volume: 227 Issue: 3 Pages: 337-344
  • DOI: 10.1007/s10014-012-0104-2
  • Peer Reviewed
• [Journal Article] Cerebral amyloid angiopathy causing large contralateral hemorrhage during surgery for lobar hemorrhage: a case report (2016)
  • Author(s): Arishima H, Neishi H, Kodera T, Kitai R, Kikuta K
  • Journal Title: J Stroke Cerebrovasc Dis Volume: 24 Issue: 3 Pages: e83-e85
  • DOI: 10.1016/j.jstrokecerebrovasdis.2014.11.015
  • Peer Reviewed
• [Journal Article] Occipital cephalocele with neural crest remnants? Radiological and pathological findings in a newborn boy (2016)
  • Author(s): Arishima H, Neishi H, Kikuta KI: Occipital cephalocele with neural crest remnants? Radiological and pathological findings in a newborn boy
  • Journal Title: Childs Nerv Syst Volume: 32 Issue: 6 Pages: 1141-1144
  • DOI: 10.1007/s00381-015-2964-3
  • Peer Reviewed
• [Presentation] Flow Insightを用いたバイパス術中の脳表血流測定～STA血流量の実測による定量補正 (2018)
  • Author(s): 菊田健一郎、川尻智士、荒井大志、東野芳史、根石拡行、常俊顕三、松田謙、有島英孝、小寺俊昭、北井隆平
  • Organizer: 第75回日本脳神経外科学会総会
• [Presentation] Effects of electrophysiological monitoring for surgery of intradural tumor of the spinal cord (2016)
  • Author(s): Kikuta K
  • Organizer: 台湾脳神経外科学科総会
  • Place of Presentation: 淡水, 台湾
  • Year and Date: 2016-12-05
  • Int'l Joint Research / Invited
• [Presentation] Essential role of sharp dissection and microanastomosis (2016)
  • Author(s): Kikuta K
  • Organizer: III International Symposium "Modern Aspects of Neurosurgery" with a Scientific and practical school of young scientists (Irkutsk-Fukui-Phoenix)
  • Place of Presentation: Irkutsk, Russia
  • Year and Date: 2016-07-28
  • Int'l Joint Research / Invited
• [Presentation] Surgical treatment of cerebral aneurysms (2016)
  • Author(s): Kikuta K
  • Organizer: III International Symposium "Modern Aspects of Neurosurgery" with a Scientific and practical school of young scientists (Irkutsk-Fukui-Phoenix)
  • Place of Presentation: Irkutsk, Russia
  • Year and Date: 2016-07-28
  • Int'l Joint Research / Invited
• [Presentation] Microsurgical resection of AVM (2016)
  • Author(s): Kikuta K
  • Organizer: III International Symposium "Modern Aspects of Neurosurgery" with a Scientific and practical school of young scientists (Irkutsk-Fukui-Phoenix)
  • Place of Presentation: Irkutsk, Russia
  • Year and Date: 2016-07-28
  • Int'l Joint Research / Invited
• [Presentation] Basic and Advanced Techniques in Bypass Surgery (2016)
  • Author(s): Kikuta K
  • Organizer: 7th Asian Congress of Neurological Surgeons
  • Place of Presentation: Surabaya, Indonesia
  • Year and Date: 2016-04-22
  • Int'l Joint Research / Invited
• [Book] 脳動静脈奇形治療のこれまでとこれから～脳神経外科のエベレスト登山 (2018)
  • Author(s): 森田明夫、伊達勲、菊田健一郎
  • Total Pages: 197
  • Publisher: Medical View社
  • ISBN: 9784758315708
• [Book] 脳神経外科手術ステップアップガイド (2016)
  • Author(s): 菊田健一郎 編著
  • Total Pages: 230
  • Publisher: 中外医薬社
• [Book] Medical View社 (2016)
  • Author(s): 森田明夫、伊達勲、菊田健一郎（編）
  • Total Pages: 180
  • Publisher: 基本開頭術と頭蓋底開頭術