Treatment study to brain tumor Using mesenchymal stem cell
| Project/Area Number |
16K10753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Amano Shinji 浜松医科大学, 医学部, 特任研究員 (70464138)
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| Co-Investigator(Kenkyū-buntansha) |
難波 宏樹 浜松医科大学, 医学部, 教授 (60198405)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 自殺遺伝子療法 / 脳腫瘍 / 間葉系幹細胞 / 脂肪由来骨髄間葉系幹細胞 / 脳神経外科学 / 遺伝子治療 |
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| Outline of Final Research Achievements |
We researched suicide gene therapy to the malignant brain tumor which has difficulty in treatment, using adipose mesenchymal stem cell(ADMSC)/ganciclovir(GCV). First mesenchymal stem cell was separated from a SD rat fat cell. Treatment was tried using ADMSC which introduced herpes simplex virus-thymidine kinase(ADMSCtk) to a rat C6 brain tumor cell. In vitro, the cell ratio of ADMSCtk:C6 also indicated anti-tumor effect in 1:8. In vivo, the tumor volume reduction effect and the extension of a survival curve were confirmed by a study using a rat. ADMSCtk/GCV treatment will be effective.
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| Academic Significance and Societal Importance of the Research Achievements |
今まで我々は一貫して、自殺遺伝子療法を用いた脳腫瘍の治療研究にあたってきた。その中で、安定的に供給できるベクターの選択が問題であった。今回、自己からも容易に採取できる脂肪細胞を用いることにより、より臨床への発展が期待できるものとなっている。脂肪細胞由来の間葉系幹細胞を用いることは、今後、他の伝子治療のベクターとして用いることができる可能性を広げるだけでなく、自己由来細胞での治療や再生医療にも応用ができる可能性がある。
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Report
(4 results)
Research Products
(1 results)
