| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
We investigated a large number of surgical ependymoma samples for the presence of RELA-fusion and TERT expression. We found RELA-fusion in the majority of supratentorial ependymomas and elevated TERT expression in all of them. To investigate the mechanism of TERT overexpression, we generated a luciferase construct with TERT promoter mutations or rs2853669 SNP. We found that the transcriptional activity of TERT promoter affected by the mutations or SNP was variable according to the length of the promoter region. In order to make a mouse model of ependymoma, we generated conditional double transgenic mouse by mating Nestin-Cre and Cag-Cas9 mouse and lentivirally introduced a RELA-fusion together with a sgRNA for Cdkn2a into the mouse brain. This resulted in development of mouse brain tumors that histologically resembles human ependymomas.
|
