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Application of human adult olfactory sphere cells as a cell source for treatment of spinal cord injury

Research Project

Project/Area Number 16K10785
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurosurgery
Research InstitutionOsaka University

Principal Investigator

Ohnishi Yu-ichiro  大阪大学, 医学系研究科, 助教 (00533811)

Co-Investigator(Kenkyū-buntansha) 岩月 幸一  大阪大学, 医学系研究科, 准教授 (80346204)
鷹羽 良平  大阪大学, 医学部附属病院, 医員 (70774686)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords脊髄損傷 / 嗅粘膜 / 神経前駆細胞 / 嗅粘膜組織 / オルファクトリースフィア細胞 / 脊髄・脊椎疾患
Outline of Final Research Achievements

Olfactory sphere cells (OSCs) are stem cells generated by culturing olfactory mucosa. Various culture conditions generate OSs that have different progenitors or stem cell characteristics. We previously reported that adult rat OSCs express oligodendrocyte progenitor cell (OPC) markers, and differentiate into oligodendrocytes in vitro and vivo. In this study we generated adult human OSCs, and analyze their characteristics. human OSCs autonomously differentiated into neurons in vitro, and had potential to be as a cell source of neurons for their own regenerative grafts, avoiding the need for immuno-suppression and ethical controversy.

Academic Significance and Societal Importance of the Research Achievements

中枢神経の再生医療では失われたニューロンをどこから得るか、またいかにして分化させるかが大きな課題である。成体中枢神経系は再生能力に乏しいが、嗅粘膜は終生ニューロンの再生が繰り返されるユニークな組織である。嗅粘膜上皮に存在するHorizontal Basal cell(HBC)は組織幹細胞であり、オルファクトリーニューロンの他、嗅粘膜を構成するボーマン嚢や支持細胞に分化する。
申請者らはHBCがオリゴデンドロサイト前駆細胞(OPC)マーカーを発現していることを見いだした。そして嗅粘膜組織より無血清培地下で、遺伝子工学的・細胞工学的手法を用いずに、OPCマーカーを発現する細胞集塊の作成に成功した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Promotion of astrocytoma cell invasion by micro RNA-22 targeting of tissue inhibitor of matrix metalloproteinase-22017

    • Author(s)
      Ohnishi YI, Iwatsuki K, Ishihara M, Ohkawa T, Kinoshita M, Shinzawa K, Fujimoto Y, Yoshimine T.
    • Journal Title

      J Neurosurg Spine

      Volume: 26(3) Issue: 3 Pages: 396-403

    • DOI

      10.3171/2016.8.spine16248

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A case of T2 radiculopathy after anterior C5-6 fusion.2016

    • Author(s)
      Takenaka T, Ohnishi YI, Oshino S.
    • Journal Title

      Journal of Surgical Case report.

      Volume: 2016(5) Issue: 5 Pages: 5-5

    • DOI

      10.1093/jscr/rjw098

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significance of the Pars Interarticularis in the Cortical Bone Trajectory Screw Technique: An In Vivo Insertional Torque Study.2016

    • Author(s)
      Ninomiya K, Iwatsuki K, Ohnishi YI, Ohkawa T, Yoshimine T.
    • Journal Title

      Asian Spine J.

      Volume: 10(5) Pages: 901-906

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Radiological Evaluation of the Initial Fixation between Cortical Bone Trajectory and Conventional Pedicle Screw Technique for Lumbar Degenerative Spondylolisthesis.2016

    • Author(s)
      Ninomiya K, Iwatsuki K, Ohnishi Y, Yoshimine T.
    • Journal Title

      Asian Spine J.

      Volume: 10(2) Issue: 2 Pages: 251-7

    • DOI

      10.4184/asj.2016.10.2.251

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Pilot Clinical Study of Olfactory Mucosa Autograft for Chronic Complete Spinal Cord Injury2016

    • Author(s)
      Iwatsuki K1, Tajima F, Ohnishi Y, Nakamura T, Ishihara M, Hosomi K, Ninomiya K, Moriwaki T, Yoshimine T.
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 56 Issue: 6 Pages: 285-292

    • DOI

      10.2176/nmc.oa.2015-0320

    • NAID

      130005157732

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Motor evoked potential and voluntary EMG activity after olfactory mucosal autograft transplantation in a case of chronic, complete spinal cord injury: case report2016

    • Author(s)
      Iwatsuki K, Tajima F, Sankai Y, Ohnishi YI, Nakamura T, Ishihara M, Hosomi K, Ninomiya K, Moriwaki T, Yoshimine T.
    • Journal Title

      Spinal cord series and cases

      Volume: Jan 7;2 Issue: 1 Pages: 15018-15018

    • DOI

      10.1038/scsandc.2015.18

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 嗅粘膜組織由来幹細胞のニューロンへの分化誘導の検討2018

    • Author(s)
      大西諭一郎
    • Organizer
      第17回日本分子脳神経外科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] CBT法椎弓根スクリューによる腰椎分離部修復と椎体間固定術2016

    • Author(s)
      大西諭一郎
    • Organizer
      第31回日本脊髄外科学会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] 内視鏡支援による片側進入-CBT法PLIF2016

    • Author(s)
      大西諭一郎
    • Organizer
      第6回低侵襲・内視鏡脊髄脊椎外科研究会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] 腰椎開窓術後に腸腰筋膿瘍と化膿性脊椎炎を呈した1例2016

    • Author(s)
      大西諭一郎
    • Organizer
      Summer Forum for Practical Spinal Surgery
    • Place of Presentation
      大阪
    • Related Report
      2016 Research-status Report
  • [Presentation] 嗅粘膜組織由来幹細胞のニューロンへの分化誘導の検討2016

    • Author(s)
      大西諭一郎
    • Organizer
      第17回日本分子脳神経外科学会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] 頭蓋底嵌入症を伴うキアリⅠ型奇形に対する大孔部減圧および硬膜外層切除による治療成績2016

    • Author(s)
      大西諭一郎
    • Organizer
      第75回日本脳神経外科学会総会
    • Place of Presentation
      福岡
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] オルファクトリースフィア細胞の生成・単離方法及び製造方法並びに該オルファクトリースフィア細胞を用いた脱随疾患治療剤及び末梢神経軸索再生増強剤の製造方法2017

    • Inventor(s)
      大西諭一郎
    • Industrial Property Rights Holder
      大西諭一郎
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Acquisition Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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