Effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration.

• Project/Area Number: 16K10808
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Tsukuba
• Principal Investigator: Hara Yuki 筑波大学, 医学医療系, 講師 (30431688)
• Research Collaborator: Ikumi Akira
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 多血小板血漿療法 / 末梢神経再生 / シュワン細胞 / 多血小板血漿 / 再生医療等製品 / 再生医療 / 再生医学

Outline of Final Research Achievements

We studied about the effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration.(1)Aquire no-clinical proof of concepts of PRP theraphy for nerve regeneration.(2)Animal examination to investigate mechanisms of PRP effect forperipheral nerve regeneration.(3)Clinical trial.Results:(1)(2)Local PRP administration increases the regenerative axon diameter and the regenerative axon number at the distal portion. PRP accelerates SC proliferation in vivo.(3)5 cases have experienced. Postoperatively, early neuropathic pain relief and good functional recovery were obtained with no PRP-related adverse events. This study demonstrates the therapeutic potential of intraoperative PRP to enhance the healing process of nerve injury in the acute phase and to decrease the neuropathic pain.

Academic Significance and Societal Importance of the Research Achievements

再生医療法の定める第3種再生医療として、末梢神経損傷に対し、新しい治療法：PRP療法を提案できた。投与により神経再生を促進することが明らかとなり、成績向上につながる治療法として期待できる。末梢神経損傷は近年、高齢者における成績不良が問題となっており、よりよい機能の再獲得を目指す新しい治療として貢献できる。

Research Products

• [Journal Article] Intraoperative Local Administration of Platelet-Rich Plasma (PRP) during Neurolysis Surgery for the Treatment of Digital Nerve Crush Injury. (2018)
  • Author(s): Ikumi A, Hara Y, Okano E, Kohyama S, Arai N, Taniguchi Y, Sugaya H, Yoshioka T, Kanamori A, Yamazaki M.
  • Journal Title: Case Rep Orthop. Volume: 1275713 Pages: 1-5
  • DOI: 10.1155/2018/1275713
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model (2017)
  • Author(s): Ikumi Akira、Hara Yuki、Yoshioka Tomokazu、Kanamori Akihiro、Yamazaki Masashi
  • Journal Title: Microsugery Volume: 38(3) Issue: 3 Pages: 300-309
  • DOI: 10.1002/micr.30263
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 自家血由来多血小板血漿（PRP）による末梢神経再生促進メカニズムの検証 (2017)
  • Author(s): 井汲彰、原友紀 ほか
  • Organizer: 第32回日本整形外科基礎学術集会
  • Invited
• [Presentation] 家兎新鮮末梢神経損傷に対する多血小板血漿（PRP）による神経再生促進効果 (2016)
  • Author(s): 原友紀、谷口悠、吉岡友和、金森章浩、山崎正志
  • Organizer: 第31回(2016年) 日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡国際会議場・福岡県・博多市
  • Year and Date: 2016-10-13