Development of vertebroplasty using autologous platelet rich plasma for osteoporotic vertebral body fracture

Research Project

Project/Area Number	16K10832
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Nagae Masateru 京都府立医科大学, 医学(系)研究科(研究院), 講師 (60604303)
Co-Investigator(Kenkyū-buntansha)	高取良太京都府立医科大学, 医学(系)研究科(研究院), 講師 (10351355) 池田巧京都府立医科大学, 医学(系)研究科(研究院), 准教授 (40453120) 外村仁京都府立医科大学, 医学(系)研究科(研究院), 助教 (70604304) 三上靖夫京都府立医科大学, 医学(系)研究科(研究院), 教授 (80360030)
Project Period (FY)	2016-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	多血小板血漿 / 骨粗鬆症 / β-TCP / 椎体形成術 / 医療・福祉 / 骨粗鬆症性椎体骨折
Outline of Final Research Achievements	The objective of this study was to investigate the effect of platelet-rich plasma (PRP) combined with gelatin β-TCP sponge on bone generation in a lumbar vertebral body defect of ovariectomized rat. After creating critical-size defects in the center of the anterior vertebral body, the defects were filled with the following materials: (1) no material (control group), (2) gelatin β-TCP sponge with PRP, and (3) gelatin β-TCP sponge with phosphate-buffered saline. In the PRP sponge group, both imaging and histological examination showed that visible osteogenesis was first induced and additional growth of bone tissue was observed in the transplanted sponge, compared with the PBS sponge group. Biomechanical test showed increased stiffness of the affected vertebral bodies in the PRP sponge group. These results indicate that PRP-impregnated gelatin β-TCP sponge is effective for facilitating bone regeneration in lumbar vertebral bone defect under osteoporotic condition.
Academic Significance and Societal Importance of the Research Achievements	これらの結果は、PRP含浸ゼラチンβ-TCPスポンジが、骨粗鬆症状態の腰椎骨欠損における骨再生を促進するのに有効であることを示している。ゼラチンβ-TCPスポンジと組み合わせたPRPは、骨粗鬆症の脊椎骨折に対する椎体形成術への新しいアプローチを開発するために有用である可能性がある。