Visualization of motor and sensory circuits in spinal cord injury using resting state functional MRI

• Project/Area Number: 16K10842
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Central Institute for Experimental Animals
• Principal Investigator: Komaki Yuji 公益財団法人実験動物中央研究所, ライブイメージングセンター, 室長代理 (10548499)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 安静時機能的MRI / 脊髄損傷 / fMRI / Diffusion MRI / MRI / resting state fMRI / functional MRI / BOLD / rs-fMRI / 神経因性疼痛 / アロディニア / 神経科学

Outline of Final Research Achievements

With this application, resting-state functional MRI in mice has been established. In addition, the motor and sensory functional circuits of neurological disorders are observed over time, and the process of functional reconstruction and compensation visualized. The results of this study will help to elucidate the pathological mechanisms of all diseases related to the brain activity network, and It is hoped that the study will be useful in evaluating the effectiveness of the treatment.

Academic Significance and Societal Importance of the Research Achievements

マウスを対象とした安静時機能的MRIを確立したことが本研究の成果である。運動および感覚機能回路の障害である神経因性疼痛モデルや脊髄損傷モデルマウスを対象として、全脳の脳活動ネットワークを初めて評価したことに学術的な意義がある。
前臨床研究として最も広く利用されるマウスの安静時機能的MRIを確立することで、精神・神経疾患に対する創薬研究へ役立てることで社会的な意義を期待する。

Research Products

• [Journal Article] Measurement of baseline locomotion and other behavioral traits in a common marmoset model of Parkinson's disease established by a single administration regimen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (2020)
  • Author(s): Ando Kiyoshi、Inoue Takashi、Hikishima Keigo、Komaki Yuji、Kawai Kenji、Inoue Ryo、Nishime Chiyoko、Nishinaka Eiko、Urano Koji、Okano Hideyuki
  • Journal Title: Behavioural Pharmacology Volume: 31 Issue: 1 Pages: 45-60
  • DOI: 10.1097/fbp.0000000000000509
  • Peer Reviewed
• [Journal Article] Common functional networks in the mouse brain revealed by multi-centre resting-state fMRI analysis (2020)
  • Author(s): Grandjean Joanes, Chika Sato, et al.
  • Journal Title: NeuroImage Volume: 205 Pages: 116278-116278
  • DOI: 10.1016/j.neuroimage.2019.116278
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characteristic cerebral structural changes identified using voxel-based morphometry in patients with post-surgical chronic myelopathic pain (2019)
  • Author(s): Horiuchi Yosuke、Tsuji Osahiko、Komaki Yuji、Fujiyoshi Kanehiro、Hikishima Keigo、Konomi Tsunehiko、Nagoshi Narihito、Watanabe Kota、Matsumoto Morio、Horiuchi Keisuke、Nakamura Masaya
  • Journal Title: Spinal Cord Volume: 58 Issue: 4 Pages: 467-475
  • DOI: 10.1038/s41393-019-0391-0
  • Peer Reviewed
• [Journal Article] Normal tension glaucoma-like degeneration of the visual system in aged marmosets (2019)
  • Author(s): Noro Takahiko、Namekata Kazuhiko、Kimura Atsuko、Azuchi Yuriko、Hashimoto Nanako、Moriya-Ito Keiko、Komaki Yuji、Lee Chia-Ying、Okahara Norio、Guo Xiaoli、Harada Chikako、Kim Euido、Nakano Tadashi、Tsuneoka Hiroshi、Inoue Takashi、Sasaki Erika、Tokuno Hironobu、Harada Takayuki
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 14852-14852
  • DOI: 10.1038/s41598-019-51281-y
  • Peer Reviewed / Open Access
• [Journal Article] Noninvasive technique to evaluate the muscle fiber characteristics using q-space imaging (2019)
  • Author(s): Hata Junichi、Nakashima Daisuke、Tsuji Osahiko、Fujiyoshi Kanehiro、Yasutake Kaori、Sera Yasushi、Komaki Yuji、Hikishima Keigo、Nagura Takeo、Matsumoto Morio、Okano Hideyuki、Nakamura Masaya
  • Journal Title: PLOS ONE Volume: 14 Issue: 4 Pages: 1-8
  • DOI: 10.1371/journal.pone.0214805
  • Peer Reviewed / Open Access
• [Journal Article] Treatment of intervertebral disk disease by administration of messenger RNA encoding a cartilage-anabolic transcription factor. (2019)
  • Author(s): C. -Y. Lin, S. T. Crowley, S. Uchida, Y. Komaki, K. Kataoka, K. Itaka
  • Journal Title: Mol. Ther. Nucleic Acids Volume: 16 Pages: 162-171
  • DOI: 10.1016/j.omtn.2019.02.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Correlative study using structural MRI and super-resolution microscopy to detect structural alterations induced by long-term optogenetic stimulation of striatal medium spiny neurons (2019)
  • Author(s): Abe Yoshifumi、Komaki Yuji、Seki Fumiko、Shibata Shinsuke、Okano Hideyuki、Tanaka Kenji F.
  • Journal Title: Neurochemistry International Volume: 125 Pages: 163-174
  • DOI: 10.1016/j.neuint.2019.02.017
  • Peer Reviewed
• [Journal Article] MR Imaging Properties of <i>ex vivo</i> Common Marmoset Brain after Formaldehyde Fixation (2019)
  • Author(s): Haga Yawara、Hata Junichi、Uematsu Akiko、Seki Fumiko、Komaki Yuji、Mizumura Mai、Nishio Marin、Kaneko Takaaki、Kishi Noriyuki、Okano Hideyuki、Furukawa Akira
  • Journal Title: Magnetic Resonance in Medical Sciences Volume: 18 Issue: 4 Pages: 253-259
  • DOI: 10.2463/mrms.mp.2018-0086
  • NAID: 130007728812
  • ISSN: 1347-3182, 1880-2206
  • Peer Reviewed / Open Access
• [Journal Article] Assessing cortical plasticity after spinal cord injury by using resting-state functional magnetic resonance imaging in awake adult mice (2018)
  • Author(s): Matsubayashi Kohei、Nagoshi Narihito、Komaki Yuji、Kojima Kota、Shinozaki Munehisa、Tsuji Osahiko、Iwanami Akio、Ishihara Ryosuke、Takata Norio、Matsumoto Morio、Mimura Masaru、Okano Hideyuki、Nakamura Masaya
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-8
  • DOI: 10.1038/s41598-018-32766-8
  • Peer Reviewed / Open Access
• [Journal Article] Optogenetic astrocyte activation evokes BOLD fMRI response with oxygen consumption without neuronal activity modulation (2018)
  • Author(s): Norio Takata, Yuki Sugiura, Keitaro Yoshida, Miwako Koizumi, Hiroshi Nishida, Kurara Honda, Ryutaro Yano, Yuji Komaki, Ko Matsui, Makoto Suematsu , Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka
  • Journal Title: Glia Volume: 印刷中 Issue: 9 Pages: 2013-2023
  • DOI: 10.1002/glia.23454
  • Peer Reviewed
• [Journal Article] Quantitative temporal changes in DTI values coupled with histological properties in cuprizone-induced demyelination and remyelination (2017)
  • Author(s): Ryutaro Yano, Junichi Hata, Yoshifumi Abe, Fumiko Seki, Keitaro Yoshida, Yuji Komaki, Hideyuki Okano, Kenji F. Tanaka
  • Journal Title: Neurochemistry International Volume: 17 Pages: 186-197
  • DOI: 10.1016/j.neuint.2017.10.004
  • Peer Reviewed
• [Journal Article] Developmental trajectories of macroanatomical structures in common marmoset brain (2017)
  • Author(s): Seki Fumiko、Hikishima Keigo、Komaki Yuji、Hata Junichi、Uematsu Akiko、Okahara Norio、Yamamoto Masafumi、Shinohara Haruka、Sasaki Erika、Okano Hideyuki
  • Journal Title: Neuroscience Volume: 364 Pages: 143-156
  • DOI: 10.1016/j.neuroscience.2017.09.021
  • Peer Reviewed
• [Journal Article] Mapping orbitofrontal-limbic maturation in non-human primates: A longitudinal magnetic resonance imaging study (2017)
  • Author(s): Uematsu Akiko、Hata Junichi、Komaki Yuji、Seki Fumiko、Yamada Chihoko、Okahara Norio、Kurotaki Yoko、Sasaki Erika、Okano Hideyuki
  • Journal Title: NeuroImage Volume: 163 Pages: 55-67
  • DOI: 10.1016/j.neuroimage.2017.09.028
  • Peer Reviewed
• [Journal Article] Elucidation of developmental patterns of marmoset corpus callosum through a comparative MRI in marmosets, chimpanzees, and humans (2017)
  • Author(s): Tomoko Sakai, Yuji Komaki, Junichi Hata, Junko Okahara, Norio Okahara, Takashi Inoue, Akichika Mikami, Mie Matsui, Kenichi Oishi, Erika Sasaki, and Hideyuki Okano
  • Journal Title: Neuroscience Research Volume: 印刷中 Pages: 25-34
  • DOI: 10.1016/j.neures.2017.04.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain (2017)
  • Author(s): Hikishima Keigo、Komaki Yuji、Seki Fumiko、Ohnishi Yasuyuki、Okano Hirotaka J.、Okano Hideyuki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 1-9
  • DOI: 10.1038/s41598-017-00148-1
  • Peer Reviewed
• [Journal Article] Functional brain mapping using specific sensory-circuit stimulation and a theoretical graph network analysis in mice with neuropathic allodynia. (2016)
  • Author(s): Komaki Y, Hikishima K, Shibata S, Konomi T, Seki F, Yamada M, Miyasaka N, Fujiyoshi K, Okano HJ, Nakamura M, Okano H.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 37802-37802
  • DOI: 10.1038/srep37802
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe (2016)
  • Author(s): Keitaro Yoshida, Yu Mimura, Ryosuke Ishihara, Hiroshi Nishida, Yuji Komaki, Tomohito Minakuchi, Tomokazu Tsurugizawa, Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka, Norio Takata
  • Journal Title: J Neurosci Methods. Volume: 274 Pages: 38-48
  • DOI: 10.1016/j.jneumeth.2016.09.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Blocking AQP4 channels impacts BOLD fMRI but not Diffusion fMRI response patterns induced by visual stimulation (2019)
  • Author(s): Komaki Yuji, Debacker Clement, Djemai Boucif, Ciobanu Luisa, Tsurugizawa Tomokazu, Le Bihan Denis
  • Organizer: ISMRM 27th Annual Meeting & Exhibition
  • Int'l Joint Research
• [Presentation] Membrane protein AQP4 for water transport influences functional MRI using 17.2 T MRI (2019)
  • Author(s): Yuji Komaki, Clement Debacker, Boucif Djemai, Luisa Ciobanu, Tomokazu Tsurugizawa, Denis Le Bihan.
  • Organizer: 第47回日本磁気共鳴医学会大会
• [Presentation] 施設紹介 Neurospin (2019)
  • Author(s): 小牧裕司
  • Organizer: 第47回日本磁気共鳴医学会大会 前臨床スタディグループ
  • Invited
• [Presentation] フランスMRI研究所 Neurospin研究報告 (2019)
  • Author(s): 小牧裕司
  • Organizer: 第47回日本磁気共鳴医学会大会 スタディグループ マイクロ・ナノ研究会
  • Invited
• [Presentation] functional MRIによる脳機能解析と薬効評価 (2019)
  • Author(s): 小牧裕司
  • Organizer: Bruker Webinar
  • Invited
• [Presentation] In vivo MRI technology to accelerate translational research for non-human primates (2017)
  • Author(s): Yuji Komaki
  • Organizer: 2017 Asia-Oceania Regional Meeting for Marmoset Research
  • Place of Presentation: Seoul
  • Year and Date: 2017-03-16
  • Int'l Joint Research / Invited
• [Presentation] Phenotyping Assay of Neuropathic Pain Models Using Resting State Functional Connectivity MRI and Graph Theoretical Analysis (2017)
  • Author(s): Yuji Komaki, Fumiko Seki, Keigo Hikishima, Masaya Nakamura, Hideyuki Okano
  • Organizer: International society for magnetic resonance in medicine (ISMRM) 25th annual meeting
  • Int'l Joint Research
• [Presentation] 橋渡し研究を加速させる低侵襲 in vivo イメージング (2017)
  • Author(s): 小牧裕司
  • Organizer: 第64回 日本実験動物学会総会
  • Invited
• [Presentation] 機能的MRIを用いた神経因性疼痛モデルマウスの全脳ネットワーク解析 (2017)
  • Author(s): 小牧裕司
  • Organizer: 生理学研究所 研究会 痛みを中心とする有害状況適応の神経戦略バイオロジー
  • Invited
• [Presentation] Atlas based whole brain analysis with the graph theoretical approach in resting state functional connectivity MRI of neuropathic pain model mice (2016)
  • Author(s): 小牧裕司
  • Organizer: 日本磁気共鳴医学会大会
  • Place of Presentation: 埼玉
  • Year and Date: 2016-09-09
• [Book] マーモセットラボマニュアル : はじめての取扱いから研究最前線まで (2018)
  • Author(s): 三木 理雅、黒滝 陽子、井上 貴史
  • Total Pages: 207
  • Publisher: アドスリー;丸善出版 (発売)
  • ISBN: 4904419782