Mechanism of meniscal healing and clinical application through SDF-1/CXCR4 signaling pathway
| Project/Area Number |
16K10871
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
Minoda Yukihide 大阪市立大学, 大学院医学研究科, 講師 (90453133)
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| Co-Investigator(Kenkyū-buntansha) |
橋本 祐介 大阪市立大学, 大学院医学研究科, 講師 (10382178)
折田 久美 (荻久美) 大阪市立大学, 大学院医学研究科, 博士研究員 (40748597)
脇谷 滋之 武庫川女子大学, 健康・スポーツ科学部, 教授 (70243243)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 半月板修復 / 再生 / SDF-1 / 半月板 / 修復 / 再生医療 / SDF1 / 半月板治癒促進 / SDF-1/CXCR4システム |
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| Outline of Final Research Achievements |
Stromal cell-derived factor 1 (SDF-1) is known to be as a chemokine vital in stem and progenitor cell recruitment for tissue repair after injury. We investigated whether intra-articular injected SDF-1 enhanced meniscal healing in rat partial meniscal defect model. The healed meniscus was evaluated 6 weeks after the resection surgery. The Pauli score by the macroscopic evaluation was improved in the SDF-1 administration group compared to the control group receiving PBS. Histological evaluation showed an increase in metachromasia with toluidine blue staining. By safranin O staining, we observed the repaired tissue surrounded by safranin O-positive extracellular matrix. It indicated that these cells seemed to undergo chondrocytic differentiation. These results suggest that intra-articular injection of SDF-1 promotes meniscal healing.
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| Academic Significance and Societal Importance of the Research Achievements |
半月板は血流に乏しいため損傷をきたすと治りにくい組織である。しかし損傷して半月板本来の衝撃吸収機能を失うと、関節軟骨に負荷がかかることから変形性関節症に至り、将来の膝関節痛の原因となる。このため現在は可能な限り半月板を温存するため、半月板縫合術という手術が選択されるが、その治癒能の乏しさから治療成績に限界があるのが現状である。
今回はSDF-1/CXCR4系という組織修復に関わるシステムを促進させることによって、半月板の良好な修復が得られることが確認できた。これは臨床に応用すれば、半月板縫合術時の治療成績の向上に役立てられる可能性がある。
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] The origin and distribution of CD68, CD163, and αSMA+ cells in the early phase after meniscal resection in a parabiotic rat model.2017
Author(s)
Terai S, Hashimoto Y, Orita K, Yamasaki S, Takigami J, Shinkuma T, Teraoka T, Nishida Y, Takahashi M, Nakamura H.
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Journal Title
Connect Tissue Res
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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