Protection mechanism against bacteria by platelet: A novel strategy for sepsis

• Project/Area Number: 16K10957
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Mie University
• Principal Investigator: Kamei Masataka 三重大学, 医学部附属病院, 教授 (60443503)
• Co-Investigator(Kenkyū-buntansha): 島岡 要 三重大学, 医学系研究科, 教授 (40281133)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 敗血症 / 血小板 / プロテオミクス / 新生児血小板 / プロテオミクス解析 / 周術期 / 止血機能 / 新生児 / platelet rich plasma / 肺血栓治療戦略 / 細菌感染防御 / Platelet Rich Plasma

Outline of Final Research Achievements

Optimization of quantitative proteome of blood was performed using ITRAQ method as a pilot study. The iTRAQ method is based on the covalent labeling of the N-terminus and side chain amines of peptides from protein digestions with tags of varying mass. Removal of albumin was needed. The blood proteins without albumin were successfully labeled by iTRAQ in our laboratory, and the samples were then pooled and usually fractionated by liquid chromatography and analyzed by tandem mass spectrometry (MS/MS). A database search is then performed using the fragmentation data to identify the labeled peptides and hence the corresponding proteins.

Academic Significance and Societal Importance of the Research Achievements

血液検体から抽出されたタンパクをアルブミン除去後にiTRAQ標識することに成功したことで、今後の血液検体の定量的プロテオミクスの道筋ができた。アルブミン除去処置をした後のタンパクサンプルのiTRAQ標識にて、100個ほどのペプチド同定、データベースとの照合によるタンパク同定を行うことができた。本研究では血小板タンパクを標的とするが、今回構築された血液検体タンパクのデータベースは、血小板の抽出効率の評価に使用できる。

Research Products

• [Journal Article] Accuracy of the ClearSight system in patients undergoing abdominal aortic aneurysm surgery. (2019)
  • Author(s): Sumiyoshi M, Maeda T, Miyazaki E, Hotta N, Sato H, Hamaguchi E, Kanazawa H, Ohnishi Y, Kamei M
  • Journal Title: J Anesth. Volume: Mar 23 Pages: 1-8
  • NAID: 40021917665
  • Peer Reviewed / Open Access
• [Journal Article] Connexins and Integrins in Exosomes (2019)
  • Author(s): Shimaoka M, Kawamoto E, Gaowa A, Okamoto T, Park EJ
  • Journal Title: Cancers (Basel) Volume: － Issue: 1 Pages: 106-106
  • DOI: 10.3390/cancers11010106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Talin-2 regulates integrin functions in exosomes (2019)
  • Author(s): Soe Zay Yar、Prajuabjinda Onmanee、Myint Phyoe Kyawe、Gaowa Arong、Kawamoto Eiji、Park Eun Jeong、Shimaoka Motomu
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 512 Issue: 3 Pages: 429-434
  • DOI: 10.1016/j.bbrc.2019.03.027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biomechanical profiles of tracheal intubation: a mannequin-based study to make an objective assessment of clinical skills by expert anesthesiologists and novice residents. (2018)
  • Author(s): Sakakura Y, Kamei M, Sakamoto R, Morii H, Itoh-Masui A, Kawamoto E, Imai H, Miyabe M, Shimaoka M.
  • Journal Title: BMC Med Educ Volume: Dec 4;18(1) Issue: 1 Pages: 293-293
  • DOI: 10.1186/s12909-018-1410-0
  • Peer Reviewed / Open Access
• [Journal Article] Exosomal regulation of lymphocyte homing to the gut (2018)
  • Author(s): Park Eun Jeong、Prajuabjinda Onmanee、Soe Zay Yar、Darkwah Samuel、Appiah Michael G.、Kawamoto Eiji、Momose Fumiyasu、Shiku Hiroshi、Shimaoka Motomu
  • Journal Title: Blood Advances Volume: 3 Issue: 1 Pages: 1-11
  • DOI: 10.1182/bloodadvances.2018024877
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Integrin and PD-1 Ligand Expression on Circulating Extracellular Vesicles in Systemic Inflammatory Response Syndrome and Sepsis (2018)
  • Author(s): Kawamoto Eiji、Masui-Ito Asami、Eguchi Akiko、Soe Zay Yar、Prajuabjinda Onmanee、Darkwah Samuel、Park Eun Jeong、Imai Hiroshi、Shimaoka Motomu
  • Journal Title: SHOCK Volume: - Issue: 1 Pages: 1-1
  • DOI: 10.1097/shk.0000000000001228
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 白血球遊走中の血管内皮バリアー機構修復メカニズム：敗血症の新規治療を目指して (2018)
  • Author(s): 亀井 政孝
  • Organizer: 日本循環制御医学会第39回総会