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Development of redox-based therapeutics against inflammation

Research Project

Project/Area Number 16K10975
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology
Research InstitutionKansai Medical University

Principal Investigator

MATSUO Yoshiyuki  関西医科大学, 医学部, 講師 (50447926)

Co-Investigator(Kenkyū-buntansha) 広田 喜一  関西医科大学, 医学部, 教授 (00283606)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords炎症 / ストレス / レドックス / チオレドキシン / 感染症 / 細胞・組織 / 蛋白質
Outline of Final Research Achievements

Accumulating evidence shows that oxidative stress is involved in the pathogenesis of a wide variety of human disorders, and the manipulation of cellular redox status has emerged as a promising therapeutic strategy to prevent an uncontrolled inflammatory response. To monitor the levels of oxidative stress, we have developed an advanced method for the rapid measurement of anti-oxidant protein thioredoxin utilizing a fully automated chemiluminescence analyzer.
We also demonstrated that cellular stress associated with protein accumulation induced the alternation of cellular redox balance in the endoplasmic reticulum, resulting in the oxidizing shift of thioredoxin-like protein TMX1. These findings suggest that the determination of TMX1 redox status could be useful to monitor the oxidative damage in the early phase of inflammatory response.

Academic Significance and Societal Importance of the Research Achievements

オンサイトでの即時検査を可能にするチオレドキシンの自動測定系を確立した。迅速かつ高感度に生体内チオレドキシンレベルを測定することが可能となり、周術期の様々な病態変化とチオレドキシンレベルの関連を明らかにすることができれば、患者の状況把握、病勢判断、治療方針の決定、予後管理などに果たす役割は大きい。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report

Research Products

(18 results)

All 2019 2018 2017 2016

All Journal Article (12 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (6 results)

  • [Journal Article] Real-time diagnostic analysis of MinION-based metagenomic sequencing in clinical microbiology evaluation: a case report2019

    • Author(s)
      Hiromasa Tanaka, Yoshiyuki Matsuo, So Nakagawa, Kenichiro Nishi, Akihisa Okamoto, Shinichi Kai, Teppei Iwai, Yoshiteru Tabata, Takeshi Tajima, Yuji Komatsu, Motohiko Satoh, Kirill Kryukov, Tadashi Imanishi, Kiichi Hirota
    • Journal Title

      JA Clin Rep

      Volume: 5 Pages: 24-24

    • DOI

      10.1186/s40981-019-0244-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION nanopore sequencer2019

    • Author(s)
      Shinichi Kai, Yoshiyuki Matsuo, So Nakagawa, Kirill Kryukov, Shino Matsukawa, Hiromasa Tanaka, Teppei Iwai, Tadashi Imanishi, Kiichi Hirota
    • Journal Title

      FEBS Open Bio

      Volume: 9 Pages: 548-557

    • DOI

      10.1002/2211-5463.12590

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Suppression of mitochondrial oxygen metabolism mediated by the transcription factor HIF-1 alleviates propofol-induced cell toxicity2018

    • Author(s)
      Sumi Chisato、Okamoto Akihisa、Tanaka Hiromasa、Kusunoki Munenori、Shoji Tomohiro、Uba Takeo、Adachi Takehiko、Iwai Teppei、Nishi Kenichiro、Harada Hiroshi、Bono Hidemasa、Matsuo Yoshiyuki、Hirota Kiichi
    • Journal Title

      Sci Rep

      Volume: 8 Pages: 8987-8987

    • DOI

      10.1038/s41598-018-27220-8

    • NAID

      120006539570

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 小胞体内レドックスバランスの維持機構2018

    • Author(s)
      松尾 禎之, 広田 喜一
    • Journal Title

      細胞

      Volume: 50 Pages: 208-209

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Propofol induces a metabolic switch to glycolysis and cell death in a mitochondrial electron transport chain-dependent manner2018

    • Author(s)
      Sumi Chisato、Okamoto Akihisa、Tanaka Hiromasa、Nishi Kenichiro、Kusunoki Munenori、Shoji Tomohiro、Uba Takeo、Matsuo Yoshiyuki、Adachi Takehiko、Hayashi Jun-Ichi、Takenaga Keizo、Hirota Kiichi
    • Journal Title

      PLoS One

      Volume: 13

    • DOI

      10.1371/journal.pone.0192796

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mitigation of inflammation using the intravenous anesthetic dexmedetomidine in the mouse air pouch model2017

    • Author(s)
      Inada Takefumi, Sumi Chisato, Hirota Kiichi, Shingu Koh, Okamoto Akihisa, Matsuo Yoshiyuki, Kamibayashi Takahiko
    • Journal Title

      Immunopharmacology and Immunotoxicology

      Volume: 39 Pages: 225-232

    • DOI

      10.1080/08923973.2017.1327964

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death2017

    • Author(s)
      Okamoto Akihisa、Sumi Chisato、Tanaka Hiromasa、Kusunoki Munenori、Iwai Teppei、Nishi Kenichiro、Matsuo Yoshiyuki、Harada Hiroshi、Takenaga Keizo、Bono Hidemasa、Hirota Kiichi
    • Journal Title

      Sci Rep

      Volume: 7 Pages: 3816-3816

    • DOI

      10.1038/s41598-017-03980-7

    • NAID

      120006364104

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anti-Inflammatory Thioredoxin Family Proteins for Medicare, Healthcare and Aging Care2017

    • Author(s)
      Yodoi Junji, Matsuo Yoshiyuki, Tian Hai, Masutani Hiroshi, Inamoto Takashi
    • Journal Title

      Nutrients

      Volume: 9 Pages: 1081-1081

    • DOI

      10.3390/nu9101081

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transmembrane thioredoxin-related protein TMX1 is reversibly oxidized in response to protein accumulation in the endoplasmic reticulum2017

    • Author(s)
      Matsuo Yoshiyuki, Hirota Kiichi
    • Journal Title

      FEBS Open Bio

      Volume: 7 Pages: 1768-1777

    • DOI

      10.1002/2211-5463.12319

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 「酸素はいつも足りていない」の生物学の建設 2016年度アルバート・ラスカー基礎医学研究賞の発表に寄せて2017

    • Author(s)
      松尾禎之、広田喜一
    • Journal Title

      LiSA

      Volume: 24 Issue: 2 Pages: 194-196

    • DOI

      10.11477/mf.3101200789

    • NAID

      40021124698

    • ISSN
      1340-8836, 1883-5511
    • Related Report
      2016 Research-status Report
  • [Journal Article] The antioxidant N-acetyl cysteine suppresses lidocaine-induced intracellular reactive oxygen species production and cell death in neuronal SH-SY5Y cells2016

    • Author(s)
      Okamoto A, Tanaka M, Sumi C, Oku K, Kusunoki M, Nishi K, Matsuo Y, Takenaga K, Shingu K, Hirota K
    • Journal Title

      BMC anesthesiology

      Volume: 16 Pages: 104-104

    • DOI

      10.1186/s12871-016-0273-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cigarette smoke reversibly activates hypoxia-inducible factor 1 in a reactive oxygen species-dependent manner2016

    • Author(s)
      Daijo H, Hoshino Y, Kai S, Suzuki K, Nishi K, Matsuo Y, Harada H, Hirota K
    • Journal Title

      Sci Rep

      Volume: 6 Pages: 34424-34424

    • DOI

      10.1038/srep34424

    • NAID

      120005850269

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 炎症が惹起する代謝リプログラミングの分子機序の探求2018

    • Author(s)
      田畑凱光、松尾禎之、広田喜一
    • Organizer
      第16回がんとハイポキシア研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ナノポアシークエンサーを用いた病原細菌の迅速同定を可能とするオンサイト解析パイプラインの確立2018

    • Author(s)
      松尾禎之、中川草、Kirill Kryukov、今西規、広田喜一
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小胞体内レドックスバランスの維持とストレス感知機構の解析:タンパク質のレドックス状態を指標としたストレスモニタリング2017

    • Author(s)
      松尾禎之、広田喜一
    • Organizer
      第70回日本酸化ストレス学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] グルタチオンシステムと連動した小胞体酸化還元酵素の活性制御およびレドックス恒常性維持の分子機構解析2017

    • Author(s)
      松尾禎之、広田喜一
    • Organizer
      第15回がんとハイポキシア研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 膜結合型酸化還元酵素TMX1のレドックス状態を指標とした小胞体ストレスのモニタリング2016

    • Author(s)
      松尾禎之、広田喜一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 膜結合型酸化還元酵素 TMX1 のレドックス状態を指標とした小胞体ストレスのモニタリング2016

    • Author(s)
      松尾禎之、広田喜一
    • Organizer
      第14回がんとハイポキシア研究会
    • Place of Presentation
      岐阜グランドホテル(岐阜県岐阜市)
    • Year and Date
      2016-11-04
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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