| Project/Area Number |
16K10976
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
千葉 義彦 星薬科大学, 薬学部, 教授 (00287848)
花崎 元彦 国際医療福祉大学, 医学部, 教授 (60379790)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 虚血再潅流傷害 / マイクロRNA / 麻酔薬 / 臓器保護作用 / 全身麻酔 / 臓器保護 / 周術期管理学 |
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| Outline of Final Research Achievements |
The aim of our study was identification of micro RNA (miRNA) relating to this organ protective effects of anesthetics. We tried to identify miRNA involved in organ injury using rat renal ischemia-reperfusion injury model. With microarray analysis and real-time PCR, we found that miR-122-5p, SLC7A1 and RhoA are involved in ischemia-reperfusion injury in rat kidney. We also found that inhalational anesthetics sevoflurane inhibits the expression of miR-122-5p and SLC7A1, suggesting sevoflurane has the protective effects on the renal ischemia-reperfusion injury.
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| Academic Significance and Societal Importance of the Research Achievements |
ラット虚血再潅流モデルから採取した腎を用い虚血再潅流傷害に関与するマイクロRNAを見出し、リアルタイムRT-PCR 法、データベース検索により腎虚血再潅流傷害の機序を解明した。これらの発現変動は吸入麻酔薬セボフルランを付加した群において抑制されており、麻酔薬が腎虚血再潅流傷害に対して保護的に作用するメカニズムを解明できた。
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