Identification of microRNA and their target genes related to organ protection by general anesthetics
Project/Area Number |
16K10976
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology
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Research Institution | Kawasaki Medical School |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
千葉 義彦 星薬科大学, 薬学部, 教授 (00287848)
花崎 元彦 国際医療福祉大学, 医学部, 教授 (60379790)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 虚血再潅流傷害 / マイクロRNA / 麻酔薬 / 臓器保護作用 / 全身麻酔 / 臓器保護 / 周術期管理学 |
Outline of Final Research Achievements |
The aim of our study was identification of micro RNA (miRNA) relating to this organ protective effects of anesthetics. We tried to identify miRNA involved in organ injury using rat renal ischemia-reperfusion injury model. With microarray analysis and real-time PCR, we found that miR-122-5p, SLC7A1 and RhoA are involved in ischemia-reperfusion injury in rat kidney. We also found that inhalational anesthetics sevoflurane inhibits the expression of miR-122-5p and SLC7A1, suggesting sevoflurane has the protective effects on the renal ischemia-reperfusion injury.
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Academic Significance and Societal Importance of the Research Achievements |
ラット虚血再潅流モデルから採取した腎を用い虚血再潅流傷害に関与するマイクロRNAを見出し、リアルタイムRT-PCR 法、データベース検索により腎虚血再潅流傷害の機序を解明した。これらの発現変動は吸入麻酔薬セボフルランを付加した群において抑制されており、麻酔薬が腎虚血再潅流傷害に対して保護的に作用するメカニズムを解明できた。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Descending necrotizing mediastinitis in a healthy young adult2018
Author(s)
Ochi Nobuaki、Wakabayashi Tokio、Urakami Atsushi、Yamatsuji Tomoki、Ikemoto Naoto、Nagasaki Yasunari、Nakagawa Nozomu、Honda Yoshihiro、Nakanishi Hidekazu、Yamane Hiromichi、Monobe Yasumasa、Akisada Takeshi、Katayama Hiroshi、Naomoto Yoshio、Takigawa Nagio
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Journal Title
Therapeutics and Clinical Risk Management
Volume: Volume 14
Pages: 2013-2017
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 門脈気腫症4例の検討2016
Author(s)
池本直人, 片山浩, 吉田悠紀子, 福田直樹, 那須敬, 落合陽子,花崎元彦
Organizer
日本臨床麻酔学会第36回大会
Place of Presentation
高知市文化プラザかるぽーと、ホテル日航高知旭ロイヤル、高知県立県民文化ホール(高知県高知市)
Year and Date
2016-11-05
Related Report
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