| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Schwann cell (SC) differentiation plays an important role in PNS myelination. However, underlying mechanisms of SC differentiation remain unclear. Here, we investigated the role of HIF1α in PNS myelination. Culturing in hypoxia or overexpression of HIF1α bearing mutation to be resistant to proteasomal degradation resulted in upregulation of myelin related gene expressions in SCs. Expression of HIF1α in SC increased during development of peripheral nerves. HIF1α stabilizing drug that inhibits prolyl hydroxylation was able to upregulate myelin protein expression and promoted myelination in culture. Transient hypoxic incubation also facilitated in vitro myelination. Expression of HIF1α was also observed in SC in peripheral nerves after injury. The number of myelinating axons was increased by a local application of a HIF1α stabilizing drug. Collectively, HIF1α might be involved in SC differentiation and peripheral myelination during development as well as regeneration after injury.
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