Bladder instillation of liposome formulation sensory neuron-specific receptor agonist for lower urinary tract dysfunction

• Project/Area Number: 16K11048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Tottori University
• Principal Investigator: HONDA Masashi 鳥取大学, 医学部, 准教授 (20362890)
• Co-Investigator(Kenkyū-buntansha): 武中 篤 鳥取大学, 医学部, 教授 (50368669) ; 齊藤 源顕 高知大学, 教育研究部医療学系基礎医学部門, 教授 (60273893)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 下部尿路機能障害 / 新規受容体 / SNSR / 膀胱内注入 / 膀胱内圧測定 / 下部尿路機能 / 排尿機能 / 薬理学 / 生理学 / リポソーム

Outline of Final Research Achievements

A novel family of G-protein-coupled receptors has been identified in rat dorsal root ganglia and named as sensory neuron-specific receptors (SNSRs). In urethane-anesthetized rats, intravesical administration of BAM8-22, a selective rat SNSR1 agonist, can inhibit the micturition reflex. Moreover, intravesica administration of BAM8-22 can ameliorate cyclophosphamide-induced bladder overactivity via suppression of capsaicin sensitive C-fiber afferent pathways.

Academic Significance and Societal Importance of the Research Achievements

本研究課題の成果から新しい受容体であるsensory neuron-specific receprots（SNSRs）を抑制する薬剤の膀胱内注入は正常排尿機能のみならず、排尿障害に対しても一定の効果をもつ可能性が示唆された。膀胱内注入は、全身投与の際に注意すべき副作用を軽減できる可能性があり、より効率的に膀胱組織に効果を発揮する事が可能となる。本成果は過活動膀胱などの排尿障害に対する新しい薬物療法を確立していく上で、重要な基盤になると考えている。

Research Products

• [Presentation] The inhibitory effects of ghrelin on micturition reflex in urethane-anesthetized rats (2018)
  • Author(s): Honda M, Tsounapi P, Kimura Y, Kawamoto B, Shimizu S, Hikita K, Shimizu T, Saito M, Takenaka A
  • Organizer: International Continence Society Annual Meeting 2018
  • Int'l Joint Research
• [Presentation] Group III metabotropic glutamate receptor-mediated regulation of micturition reflex in urethane-anesthetized rats (2017)
  • Author(s): Honda M, Kimura Y, Kawamoto B, Tsounapi P, Hikita K, Shimizu S, Shimizu T, Saito M, Takenaka A
  • Organizer: American Urological Association Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] Intravesical administration of sensory neuron-specific receptor agonist suppresses cyclophosphamide-induced bladder overactivity (2016)
  • Author(s): Honda M, Yoshimura N, Kawamoto B, Tsounapi P, Shimizu S, Morizane S, Hikita K, Muraoka K, Shimizu T, Sejima T, Saito M, Chancellor MB, Takenaka A
  • Organizer: American Urological Association Annual Meeting 2016
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research