| Project/Area Number |
16K11058
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
東原 英二 杏林大学, 医学部, 特任教授 (00092312)
長尾 静子 藤田医科大学, 疾患モデル教育研究施設, 教授 (20183527)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
|---|
| Keywords | polycystic kidney / pericyte / 内在性レトロウイルス / Gag遺伝子 / HIF / 多発性嚢胞腎 / PCKラット / ペリサイト / NG2 / HIF-1 / ベンゾイソフラノン誘導体 / CD31 / ＰＣＫラット / ベンゾイソフラノン化合物 / 血管保護 / マクロファージ |
|---|
| Outline of Final Research Achievements |
Using PCK rat model and the novel isobenzofuranone compound, NK0070, for 15 weeks administration, the morphological findings of PCK kidneys demonstrated the amelioration of narrowing and dilatation of descending vasa recta (DVR) between the outer medulla and the base of papilla, in which narrowing and dilatation of DVR were minimized and NG2 of pericyte marker was stained keeping around DVR. Furthermore, the lesions of glomerular capillary lumen of juxtamedullary glomeruli such as dilatation and hyalinosis was also ameliorated. Mlana of Gag genes of endogenous retrovirus, in addition to TET enzyme and HIF-1, of NK0070-treated PCK rat kidneys were studies.
|
| Academic Significance and Societal Importance of the Research Achievements |
新規イソベンゾフラノン低分子化合物、NK0070により血管新生、血管保護にかかわるpericytes とGalectin-3、転写因子HIF-1にくわえLTR(Long-terminal Repeat)をもつ内在性レトロウイルスGag遺伝子:RTL1, Mlanaの関与を検討した。臓器障害への有用な修復に向かわせる宿主の応答機構としてLTRを有す内在性レトロウイルスの存在と臨床上の意義付けを与え、新規化合物創薬などふくめ新しい修復再生治療の手がかりとなる可能性がある。
|
