The condition change by neural regulation modified by glia cells.

• Project/Area Number: 16K11060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Gihu University of Medical Science (2018-2019); Kwansei Gakuin University (2016-2017)
• Principal Investigator: Matsuyoshi Hiroko 岐阜医療科学大学, 保健科学部, 准教授 (10448772)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 膀胱炎 / ラマン分光学 / 慢性膀胱炎モデル / ラマン分光法 / モデル動物 / ラマン分光 / 膀胱知覚神経 / 衛星細胞

Outline of Final Research Achievements

The spectra changes between the BPS/IC model and control animals were detected and the spectral alteration was affected by the effect of property change of urothelium; structure proteins. The Raman technique used this sturdy can be applied the living animal analysis without pretreatment noninvasively. Hence, the Raman technique has a possibility as a detection system of animal condition change by neural regulation modified by glia cells.

Academic Significance and Societal Importance of the Research Achievements

今研究では、急性・慢性膀胱炎モデルラット膀胱からラマンスペクトルを検出することが可能であり、ラマン分光法の使用により、免疫組織学的実験では明らかにできない構造変化の原因を推測することが出来ることを明らかにした。加えて、今回使用したラマンプローブはヒトの膀胱鏡との併用が可能な大きさである。故に、ラマン分光法の応用により、間質性膀胱炎や膀胱癌の非侵襲的な診断技術を確立できる可能性がある。

Research Products

• [Journal Article] ラマン分光を応用した膀胱病態評価 (2019)
  • Author(s): 松吉ひろ子、竹谷皓規、佐藤英俊
  • Journal Title: レーザー研究 Volume: 47 Pages: 107-110
  • NAID: 130008074035
  • Peer Reviewed
• [Presentation] Raman for detection of the pathological change of the bladder (2018)
  • Author(s): Matsuyoshi H、Takama S、Tanioka T、Sato H
  • Organizer: SPEC 2018
  • Int'l Joint Research
• [Presentation] Raman study to explore a new discriminate method of bladder condition (2017)
  • Author(s): Matsuyoshi Hiroko, Taketani Akinori, Iwasaki Yuki, Yoshimura Naoki, Sato Hidetoshi
  • Organizer: ICS2017
  • Int'l Joint Research
• [Presentation] ラマン分光を応用した膀胱病態評価・分析技術の開発 (2017)
  • Author(s): 松吉ひろ子、高間柊平、佐藤英俊
  • Organizer: 第15回医用分光学研究会
• [Presentation] Raman study for development of a new discriminate method of bladder conditions of Bladder pain syndrome/Interstitial cystitis (BPC/IC) model rat (2016)
  • Author(s): Hiroko Matsuyoshi, Akinori Taketani, Yuki Iwasaki, Naoki Yoshimura, Hidetoshi Sato
  • Organizer: Japan-Taiwan Medical Spectroscopy International Symposium
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2016-12-04
  • Int'l Joint Research
• [Presentation] Raman study for detecting the change of property of DRG neurons innervating the urinary bladder (2016)
  • Author(s): Hiroko Matsuyoshi, Kosuke Hashimoto, Naoya Ogawa, Akinori Taketani, Naoki Yoshimura, Hidetoshi Sato
  • Organizer: Spec 2016
  • Place of Presentation: モントリオール（カナダ）
  • Year and Date: 2016-06-25
  • Int'l Joint Research