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Diversity of Neisseria gonorrhoeae strains in the host response

Research Project

Project/Area Number 16K11061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Shimuta Ken  国立感染症研究所, 細菌第一部, 主任研究官 (40370960)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords性感染症 / 淋菌感染症 / STI
Outline of Final Research Achievements

The differences of individual Neisseria gonorrhoeae strains about host response were examined. The same genotype, MLST, was set as the same group, and five groups were proposed as pre-dominant strains in Japan. I confirmed the differences between the groups. When the growth patterns of the strains were examined under some liquid culture conditions, the strains belonging to the MLST7359 showed a different pattern from the strains belonging to other lineage under the condition using BHI medium. The expression of thes LOS, which affects inflammatory of host response, and cell adhesion to cultured cells was confirmed, differences were observed between N. gonorrhoeae strains, but no lineages-dependent differences were observed.

Academic Significance and Societal Importance of the Research Achievements

本課題の対象となる淋菌株の本邦における状況を明らかにするために、分離株の遺伝子型(MLST型)を決定した。その結果、短期間において流行株の変化が起きていることが分かった。適切な淋菌感染症における治療指針を策定するには、その時代に則したものが必要であり、そのためには対象となる分離株の最新の性状を把握することが重要であり、今回得られた情報はその策定を行う上で貢献できるものと考えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018 2017 2016

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria Gonorrhoeae penA-60.0012019

    • Author(s)
      Ken Shimuta, Shu-Ichi Nakayama, Hideyuki Takahashi, Makoto Ohnishi
    • Journal Title

      Antimicrob Agents Chemother .

      Volume: 64 Issue: 1

    • DOI

      10.1128/aac.01663-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clonal expansion and spread of the ceftriaxone-resistant Neisseria gonorrhoeae strain FC428, identified in Japan in 2015, and closely related isolates2019

    • Author(s)
      203)Lee K, Nakayama SI, Osawa K, Yoshida H, Arakawa S, Furubayashi KI, Kameoka H, Shimuta K, Kawahata T, Unemo M, Ohnishi M.
    • Journal Title

      J Antimicrob Chemother

      Volume: 74 Issue: 7 Pages: 1812-1819

    • DOI

      10.1093/jac/dkz129

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Real-Time PCR Assay for the Detection of a penA Mutation Associated with Ceftriaxone Resistance in Neisseria gonorrhoeae.2019

    • Author(s)
      Shimuta K, Igawa G, Yasuda M, Deguchi T, Nakayama SI, Ohnishi M.
    • Journal Title

      J Glob Antimicrob Resist

      Volume: in press Pages: 46-49

    • DOI

      10.1016/j.jgar.2019.02.011

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genomic surveillance of Neisseria gonorrhoeae to investigate the distribution and evolution of antimicrobial-resistance determinants and lineages2018

    • Author(s)
      Yahara Koji、Nakayama Shu-ichi、Shimuta Ken、Lee Ken-ichi、Morita Masatomo、Kawahata Takuya、Kuroki Toshiro、Watanabe Yuko、Ohya Hitomi、Yasuda Mitsuru、Deguchi Takashi、Didelot Xavier、Ohnishi Makoto
    • Journal Title

      Microbial Genomics

      Volume: 4 Issue: 8 Pages: 1-13

    • DOI

      10.1099/mgen.0.000205

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neisseria cinerea with High Ceftriaxone MIC Is a Source of Ceftriaxone and Cefixime Resistance-Mediating penA Sequences in Neisseria gonorrhoeae.2018

    • Author(s)
      Igawa G, Yamagishi Y, Lee KI, Dorin M, Shimuta K, Suematsu H, Nakayama SI, Mikamo H, Unemo M, Ohnishi M
    • Journal Title

      Antimicrob Agents Chemother

      Volume: 62 Issue: 3

    • DOI

      10.1128/aac.02069-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Antimicrobial susceptibility of Haemophilus influenzae strains isolated from the urethra of men with acute urethritis and/or epididymitis.2017

    • Author(s)
      Deguchi T, Ito S, Hatazaki K, Horie K, Yasuda M, Nakane K, Mizutani K, Tsuchiya T, Yokoi S, Hanaoka N, Shimuta K, Ohnishi M, Muratani T, Nakano M
    • Journal Title

      J Infect Chemother

      Volume: 23 Issue: 11 Pages: 804-807

    • DOI

      10.1016/j.jiac.2017.05.009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conjunctivitis Caused by a Strain of <i>Neisseria gonorrhoeae</i> That Was Less Susceptible to Ceftriaxone2017

    • Author(s)
      Iwata A, Shimuta K, Ohnishi M
    • Journal Title

      Internal Medicine

      Volume: 56 Issue: 11 Pages: 1443-1445

    • DOI

      10.2169/internalmedicine.56.7656

    • NAID

      130006857224

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Haemophilus influenzae Isolated From Men With Acute Urethritis: Its Pathogenic Roles, Responses to Antimicrobial Chemotherapies, and Antimicrobial Susceptibilities.2017

    • Author(s)
      Ito S, Hatazaki K, Shimuta K, Kondo H, Mizutani K, Yasuda M, Nakane K, Tsuchiya T, Yokoi S, Nakano M, Ohinishi M, Deguchi T.
    • Journal Title

      Sexually Transmitted Diseses

      Volume: 44 Issue: 4 Pages: 205-210

    • DOI

      10.1097/olq.0000000000000573

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] New ceftriaxone- and multidrug-resistant Neisseria gonorrhoeae strain with a novel mosaic penA gene isolated in Japan2016

    • Author(s)
      Nakayama SI, Shimuta K, Furubayashi KI, Kawahata T, Unemo M, Ohnishi M
    • Journal Title

      Antimicrob Agents Chemother

      Volume: 60 Issue: 7 Pages: 4339-4341

    • DOI

      10.1128/aac.00504-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] SNPタイピングによるセフトリアキソン耐性淋菌株の保持するpenA 遺伝子のLAMP法による検出2020

    • Author(s)
      志牟田 健
    • Organizer
      第12回LAMP研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 2018年にわが国で分離された淋菌の薬剤感受性報告2019

    • Author(s)
      安田満、志牟田健、中山周一、小林寅喆、大澤佳代、陣内理生、三宅啓文、浜砂良一、荒川創一、大西真
    • Organizer
      日本性感染症学会 第32回学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 淋菌pneA 60.001遺伝子のLAMP法による検出2019

    • Author(s)
      志牟田 健、中山周一、高橋英之、大 西 真
    • Organizer
      日本性感染症学会 第32回学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] CTRX耐性N. gonorrhoeaeおよびCTRX低感受性N. subflavaに対するpenA遺伝子解析2018

    • Author(s)
      金坂伊須萌、山根夏枝、伊與田貴子、天野綾子、小山英明、松本 哲、勝瀬(金山) 明子、志牟田健、大西 真、小林寅喆
    • Organizer
      日本性感染症学会第31回学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 2015~2017年にわが国で分離された淋菌の薬剤感受性報告2018

    • Author(s)
      安田満、志牟田健、中山周一、小林寅喆、大澤佳代、陳内理生、三宅啓文、濵砂良一、荒川創一、大西 真
    • Organizer
      日本性感染症学会第31回学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] セフトリアキソン耐性淋菌特異的リアルタイムPCR系の構築2017

    • Author(s)
      志牟田 健、井川ジーン、中山周一、大 西 真
    • Organizer
      日本性感染症学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 大阪府内において分離された淋菌株の薬剤感受性率の推移と多剤耐性の傾向2017

    • Author(s)
      川畑拓也、古林敬一、亀岡 博、安本亮二、中山周一、志牟田健、大西 真
    • Organizer
      日本性感染症学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 種を超えた遺伝子伝播―耐性遺伝子プールの存在2017

    • Author(s)
      志牟田 健
    • Organizer
      第66回日本感染症学会東日本地方学術集会、第64回日本化学療法学会東日本支部総会合同学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Characterization of azithromycin resistant Neisseria gonorrhoeae strains isolated in Kyoto and Osaka, Japan, 2010-20152016

    • Author(s)
      Ken Shimuta, Shu-ichi Nakayama, Tomoko Morita-Ishihara, Makoto Ohnishi
    • Organizer
      19th IUSTI Asia-Pacific Conference
    • Place of Presentation
      Okayama
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2021-02-19  

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