Role of endothelial glycocalyx in transplant immune responses; a mechanism of different susceptibility for rejection in various blood vessels
| Project/Area Number |
16K11070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Showa University |
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Principal Investigator |
Honda Kazuho 昭和大学, 医学部, 教授 (10256505)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 臓器移植 / 拒絶反応 / 移植片対宿主反応 / 血管内皮 / グリコキャリックス / 低真空走査型電子顕微鏡 / 腎移植 / 骨髄移植 / GVHD |
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| Outline of Final Research Achievements |
Endothelial glycocalyx (GCX) is a thin layer covering endothelial cell membrane and regulates various biological reactions in the blood vessels. To investigate the relationship between GCX and vascular immunological responses in transplant organ, we established a simple method for 3-dimensional visualization of endothelial GCX by scanning electron microscopy using formalin fixed paraffin embedded (FFPE) samples with immersion of Alcian blue containing fixative. After periodic acid methenamine silver (PAM) staining of the section, the Alcian blue particles attached to the endothelial GCX are visualized clearly by SEM. This method will enable us to evaluate the endothelial GCX alterations in organ transplant rejection and graft versus host disease.
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| Academic Significance and Societal Importance of the Research Achievements |
血管内皮のグリコキャリックスは，1ミクロン以下の壊れやすい薄層で，通常の標本作成手技では喪失しやすく，さらに可視化には血管内色素潅流などが必要で，ヒト臨床検体への応用は困難であった．今回確立したアルシアン青含有固定液と銀染色による血管内皮グリコキャリックスの3次元的可視化法は，浸漬固定材料にも応用が可能で，今後ヒトの臨床検体を用いて移植拒絶反応や移植片対宿主反応の病態の解明や新たな治療の確立に役立つことが期待できる．
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Report
(5 results)
Research Products
(80 results)
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[Journal Article] Monoclonal immunoglobulin G deposits on tubular basement membrane in renal allograft: is this significant for chronic allograft injury?2019
Author(s)
Sawada A, Kawanishi K, Horita S, Omoto K, Okumi M, Shimizu T, Taneda S, Fuchinoue S, Ishida H, Honda K, Hattori M, Tanabe K, Koike J, Nagashima Y, Nitta K.
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Journal Title
Nephrol Dial Transplant
Volume: 34
Issue: 4
Pages: 711-717
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Successful entecavir plus prednisolone treatment for hepatitis B virus-associated membranoproliferative glomerulonephritis: A case report.2019
Author(s)
Kataoka H, Mochizuki T, Akihisa T, Kawasoe K, Kawachi K, Makabe S, Sawada A, Manabe S, Sato M, Amemiya N, Mitobe M, Akanuma T, Ito Y, Inoue T, Suzuki T, Matsui K, Moriyama T, Horita S, Ohara M, Honda K, Nitta K.
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Journal Title
Medicine (Baltimore)
Volume: 98
Issue: 2
Pages: e14014-e14014
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pituitary adenylate cyclase-activating polypeptide promotes eccrine gland sweat secretion.2017
Author(s)
Sasaki S, Watanabe J, Ohtaki H, Matsumoto M, Murai N, Nakamachi T, Hannibal J, Fahrenkrug J, Hashimoto H, Watanabe H, Sueki H, Honda K, Miyazaki A, Shioda S.
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Journal Title
Br J Dermatol.
Volume: 176
Issue: 2
Pages: 413-422
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Vascular endothelial cell injury is an important factor in the development of encapsulating peritoneal sclerosis in long-term peritoneal dialysis patients.2016
Author(s)
Tawada M, Ito Y, Hamada C, Honda K, Mizuno M, Suzuki Y, Sakata F, Terabayashi T, Matsukawa Y, Maruyama S, Imai E, Matsuo S, Takei Y.
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Journal Title
PLoS One
Volume: 11
Issue: 4
Pages: e0154644-e0154644
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Interstitial fibrosis is the critical determinant of impaired renal function in transplant glomerulopathy.2016
Author(s)
Toki D, Inui M, Ishida H, Okumi M, Shimizu T, Shirakawa H, Omoto K, Unagami K, Setoguchi K, Koike J, Honda K, Yamaguchi Y, Tanabe K.
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Journal Title
Nephrology (Carlton)
Volume: 21 Suppl 1
Issue: S1
Pages: 20-25
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Therapeutic effects and mechanism of conditioned media from human mesenchymal stem cells on anti-GBM glomerulonephritis in WKY rats.2016
Author(s)
Iseri K, Iyoda M, Ohtaki H, Matsumoto K, Wada Y, Suzuki T, Yamamoto Y, Saito T, Hihara K, Tachibana S, Honda K, Shibata T.
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Journal Title
Am J Physiol Renal Physiol.
Volume: 10
Issue: 11
Pages: 1152-1152
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Suppression of Spinal Cord Injury by Human Mesenchymal Stem/Stromal Cells (hMSCs) via Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Mice.2016
Author(s)
Ohtaki H, Tsumuraya T, Sato A, Miyamoto K, Yoshikawa A, Watanabe J, Hiraizumi Y, Dohi K, Hashimoto H, Honda K.
Organizer
The 39th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
パシフィコ横浜,横浜
Year and Date
2016-07-20
Related Report
Int'l Joint Research
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