| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Endothelial glycocalyx (GCX) is a thin layer covering endothelial cell membrane and regulates various biological reactions in the blood vessels. To investigate the relationship between GCX and vascular immunological responses in transplant organ, we established a simple method for 3-dimensional visualization of endothelial GCX by scanning electron microscopy using formalin fixed paraffin embedded (FFPE) samples with immersion of Alcian blue containing fixative. After periodic acid methenamine silver (PAM) staining of the section, the Alcian blue particles attached to the endothelial GCX are visualized clearly by SEM. This method will enable us to evaluate the endothelial GCX alterations in organ transplant rejection and graft versus host disease.
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