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The analysis of roles of NLRP7 in molar pregnancies using NLRP7-deleted HTR-8/SVneo cells.

Research Project

Project/Area Number 16K11120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKio University

Principal Investigator

Maehara Kayoko  畿央大学, 健康科学部, 教授 (80421311)

Co-Investigator(Kenkyū-buntansha) 祐實 泰子  畿央大学, 健康科学部, 講師 (80425454)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords反復胞状奇胎 / NLRP7遺伝子 / ゲノム編集 / 遺伝子 / 細胞・組織 / 産科学
Outline of Final Research Achievements

Hydatidiform moles (HMs) are abnormal pregnancies with trophoblastic hyperplasia. Recent studies have shown that mutations of NLRP7 gene are associated with recurrent hydatidiform moles (RHMs), although the mechanism underlying molar pregnancies affected by the mutations of NLRP7 remains unknown. To investigate the effects of NLRP7 on cell growth in trophoblasts, we deleted NLRP7 from HTR-8/SVneo cells, a trophoblast cell line, by the CRISPR/Cas9 system. To minimize the off-target effect, D10A mutant nickase version of Cas9 (Cas9D10A) combined with guide RNA (gRNA) pairs having high sequence-specificity evaluated by our developing tool (fcrisprt) was applied. Of 50 clones screened, NLRP7 was successfully disrupted in two clones; clone 1 and clone 2 obtained biallelic 20 bp-deletion and 2 bp-insertion within exon 4 of NLRP7 gene, respectively.

Academic Significance and Societal Importance of the Research Achievements

遺伝子の機能を解析には、細胞やモデル生物(個体)を利用する方法があり、マウスなどのげっ歯類が利用されている。しかしNLPR7遺伝子はげっ歯類に保存されておらず、ノックアウトマウスを用いた解析などができない。近年開発されたゲノム編集を利用することで、NLRP7遺伝子の破壊をヒトの細胞で行い、疾患モデル細胞を作成できた。反復胞状奇胎の病態形成を研究するための有用なツールになると考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2015 Other

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (1 results) (of which Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Elucidation of the developmental mechanism of ovarian mature cystic teratomas using B allele-frequency plots of single nucleotide polymorphism array data2018

    • Author(s)
      Usui Hirokazu、Nakabayashi Kazuhiko、Kaku Hiroshi、Maehara Kayoko、Hata Kenichiro、Shozu Makio
    • Journal Title

      Genes, Chromosomes and Cancer

      Volume: 57 Issue: 8 Pages: 409-419

    • DOI

      10.1002/gcc.1

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A versatile technology for targeted genome editing has an impact on our lives.2017

    • Author(s)
      前原 佳代子
    • Journal Title

      畿央大学紀要

      Volume: 14 Issue: 1 Pages: 1-8

    • DOI

      10.24482/00000009

    • NAID

      120006323665

    • URL

      https://kio.repo.nii.ac.jp/records/18

    • Year and Date
      2017-06-30
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Nonsense Mutation in the NLRP7 Gene Associated with Recurrent Hydatidiform Mole.2015

    • Author(s)
      Ito Y, Maehara K, Kaneki E, Matsuoka K, Sugahara N, Miyata T, Kamura H, Yamaguchi Y, Kono A, Nakabayashi K, Migita O, Higashimoto K, Soejima H, Okamoto A, Nakamura H, Kimura T, Wake N, Taniguchi T, Hata K.
    • Journal Title

      Gynecol Obstet Invest

      Volume: Epub ahead of print Issue: 4 Pages: 1-6

    • DOI

      10.1159/000441780

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ヒト早老症モデル細胞の開発2018

    • Author(s)
      前原佳代子
    • Organizer
      第36回日本ヒト細胞学会学術集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Book] 学生と考える生命倫理[第2版]2018

    • Author(s)
      金子 章道、金内 雅夫、河野 由美、島 恒生、前原 佳代子、堀江 尚子、藤澤 弘枝、福本 貴彦、山本 隆、森岡 周、西井 康惠、訓覇 秋麿、細越 寛樹、小野 尚香、安井 義和
    • Total Pages
      232
    • Publisher
      ナカニシヤ出版
    • ISBN
      9784779512216
    • Related Report
      2017 Research-status Report
  • [Remarks] 畿央大学教員紹介データベース 前原佳代子

    • URL

      http://webinfo.kio.ac.jp/kio1/view2.asp?msg_no=275

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] 畿央大学ホームページ

    • URL

      http://www.kio.ac.jp/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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