| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We developed artificial middle ear mucosal sheets from cultured middle ear mucosa that was taken from middle ear cavity of a patient who was not involved in a middle ear disease by using the temperature-responsive culture dish. Then, we developed tympanic membrane epithelium sheets consist of extra cellular matrix and keratinocyte that was taken from external ear canal of the same patient. After that, we finally manufactured an artificial tympanic membrane by fusion of artificial middle ear mucosal sheets and tympanic membrane epithelium sheets. We confirmed this artificial tympanic membrane was resembled normal tympanic membrane from the viewpoint of pathohistologic characteristics by immunohistochemical staining and electron microscope findings. Although, we attempted to develop an experimental middle ear cholesteatoma model by using the artificial tympanic membrane, it was impossible to develop it only by the stimulation of some chemical mediators and cytokines.
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