Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA

• Project/Area Number: 16K11214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: The University of Tokushima
• Principal Investigator: KITAMURA Yoshiaki 徳島大学, 大学院医歯薬学研究部(医学域), 講師 (60380028)
• Co-Investigator(Kenkyū-buntansha): 武田 憲昭 徳島大学, 大学院医歯薬学研究部(医学域), 教授 (30206982) ; 水口 博之 大阪大谷大学, 薬学部, 教授 (40247838)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナローバンドUVB / アレルギー性鼻炎 / 光線療法 / ヒスタミンH1受容体 / 狭帯域中波紫外線 / ヒスタミンH１受容体 / アレルギー性鼻炎モデルラット / LED / アレルギー

Outline of Final Research Achievements

Irradiation with low dose of 310nm narrowband-ultraviolet B (NB-UVB)wavelength-specifically, dose-dependently, reversibly suppressed phorbol-12-myristate-13-acetate-induced up-regulation of histamine H1 receptor (H1R) mRNA without induction of apoptosis in HeLa cells.Intranasal irradiation with 310 nm of NB-UVB dose-dependently and reversibly suppressed toluene 2,4-diisocyanate(TDI)-induced nasal symptoms and up-regulation of H1R mRNA without inducing DNA damage and apoptosis in the nasal mucosa of TDI-sensitized rats. We can expect that phototherapy with NB-UVB will be effectively and safely applied for the treatment of allergic rhinitis.

Academic Significance and Societal Importance of the Research Achievements

ナローバンドUVB光線療法は、308～313nmの狭帯域波長の紫外線を用いた光線療法であり、皮膚疾患の治療にすでに臨床応用されている。ナローバンド紫外線を発光するLEDを開発し照射機器を小型化することで、アレルギー性鼻炎に対しての応用が期待できるようになった。そこで、アレルギー性鼻炎動物モデルラットを用いて、ナローバンドUVB光線療法の効果を検討すると、アレルギー性鼻炎症状と鼻粘膜のヒスタミンH1受容体遺伝子発現の亢進を照射量依存性にアポトーシスを誘導することなく抑制することができた。鼻炎に対する効果と持続時間、そして安全性について確認することができ、臨床応用への期待が高まった。

Research Products

• [Journal Article] Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats (2018)
  • Author(s): Fujii Tatsuya、Kitamura Yoshiaki、Mizuguchi Hiroyuki、Okamoto Kentaro、Sanada Nanae、Yamada Takuya、Sugiyama Manabu、Michinaga Shotaro、Kitayama Mika、Fukui Hiroyuki、Takeda Noriaki
  • Journal Title: Journal of Pharmacological Sciences Volume: 138 Issue: 1 Pages: 54-62
  • DOI: 10.1016/j.jphs.2018.08.011
  • Peer Reviewed / Open Access
• [Presentation] Effects of low dose irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA in rat model of allergic rhinitis (2018)
  • Author(s): Seiichiro Kamimura, Yoshiaki Kitamura, Tatsuya Fujii, Hiroyuki Mizuguchi, Hiroyuki Fukui, Noriaki Takeda
  • Organizer: 27th Congress of the European Rhinologic Society
  • Int'l Joint Research
• [Presentation] Molecular mechanism of PMA-induced up-regulation of interleukin-33 gene expression and the effect of Sho-seiryu-to on IL-33 and histamine H1 receptor gene expression (2016)
  • Author(s): Yoshiaki Kitamura, Takako Esu, Hiroyuki Mizuguchi, Shiho Naniwa,Tatsuya Fujii, Noriaki Takeda, Hiroyuki Fukui
  • Organizer: 26th Congress of the European Rhinologic Society
  • Place of Presentation: Stockholm(Sweden)
  • Year and Date: 2016-07-03
  • Int'l Joint Research
• [Book] JOHNS (2016)
  • Author(s): 北村嘉章、藤井達也、武田憲昭
  • Total Pages: 1788
  • Publisher: 東京医学社