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Role of epithelial-mesenchymal transition in maintenance and repair of vocal cord

Research Project

Project/Area Number 16K11230
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionKyoto University

Principal Investigator

Kitamura Morimasa  京都大学, 医学研究科, 助教 (60543262)

Co-Investigator(Kenkyū-buntansha) 大森 孝一  京都大学, 医学研究科, 教授 (10233272)
平野 滋  京都府立医科大学, 医学(系)研究科(研究院), 教授 (10303827)
楯谷 一郎  京都大学, 医学研究科, 講師 (20526363)
岸本 曜  京都大学, 医学研究科, 特定病院助教 (80700517)
Research Collaborator Kabashima Kenji  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords上皮間葉移行 / 声帯 / トランスジェニックマウス / マウス / 傷害 / 発声・分化
Outline of Final Research Achievements

Experiments were performed using transgenic mice in which the K5 Cre strain and the CAG-td Tomato strain were crossed. As a result, the presence of epithelial-mesenchymal transition cells was satisfied in all of 1) existence in the lamina propria of vocal cord, 2) positive Tomato, 3) negative E-cadherin, and 4) positive Vimentin.
The vocal cord injury of the transgenic mice was performed, and the larynx was extracted after 1, 3, 5 and 14 days. The cells of the lamina propria lamina were evaluated by immunostaining. As a result, there were no epithelial-mesenchymal transition cells in the intact vocal cords and vocal cords 1, 3 and 5 days after injury, but epithelial mesenchymal transition cells were recognized at a ratio of 1 cell in 5-6 sections after 14 days of injury.

Academic Significance and Societal Importance of the Research Achievements

上皮間葉転換後も上皮由来の細胞が追跡できるトランスジェニックマウスを用いた声帯の研究は世界においても他に類を見ない研究であり、本実験で上皮由来細胞が声帯粘膜固有層に移行し間葉細胞へ分化していることが確認された。これは上皮由来幹細胞が多分化能を示す証拠となる。声帯創傷治癒において重視されるべき治療ターゲットが明らかになり、声帯瘢痕に対する治療戦略の確立に寄与することが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis2017

    • Author(s)
      Suzuki Ryo、Katsuno Tatsuya、Kishimoto Yo、Nakamura Ryosuke、Mizuta Masanobu、Suehiro Atsushi、Yamashita Masaru、Nakamura Tatsuo、Tateya Ichiro、Omori Koichi
    • Journal Title

      The Laryngoscope

      Volume: 128 Issue: 4

    • DOI

      10.1002/lary.26959

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Localization of tight junction protein claudin subtypes in the vocal fold epithelium.2018

    • Author(s)
      Suzuki R, Katsuno T, Kishimoto Y, Mizuta M, Suehiro A, Yamashita M, Tateya I, Omori K.
    • Organizer
      The 139th American Laryngological Association.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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