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Basic technology development of corneal regenerative medicine using RSPO1

Research Project

Project/Area Number 16K11326
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Nagata Maho  京都府立医科大学, 医学部附属病院, 客員講師 (40614102)

Research Collaborator Fullwood Nigel  Lancaster University, Research directory
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRSPO1 / 角膜混濁 / Wnt / 炎症 / 創傷治癒 / 角膜上皮 / S100A8/A9 / カルシウム沈着 / R-spondin1 / タイトジャンクション / S100A8 / S100A9 / 角膜 / Wntシグナル / 眼科学
Outline of Final Research Achievements

Corneal transparency is necessary to maintain visual function, but the mechanism to keep its transparency remains unclear. We found that RSPO1 gene-deficient mouse gradually occurs corneal opacity and deposit. In this mouse, increased inflammation and delayed wound healing were found. Moreover, we found that the barrier function of the corneal epithelium was poor in this mouse and the deposits within cornea contained calcium. Gene expression analysis of the corneal epithelium revealed that the calcium-binding proteins S100A8/A9 were remarkably up-regulated in RSPO1 gene-deficient mouse. We suppose that RSPO1 originally keeps the barrier function of corneal epithelium, suppresses S100A8/A9 expression, and prevents accumulating calcium deposits. We conclude that RSPO1 is one of the key to elucidate the mechanism of maintaining the corneal transparency.

Academic Significance and Societal Importance of the Research Achievements

本研究は、角膜の透明性維持機構の一部を解明するものである。角膜の透明性が失われる疾患で、とくに炎症が遷延したり創傷治癒遅延がおこる素因があると、角膜移植を行っても最終的に透明性維持が難しく視力が回復しない場合がある。角膜の透明性維持機構を解明することで、今まで治癒することが難しかった角膜疾患の治療に役立てられる可能性がある。本研究では、RSPO1が炎症を制御し創傷治癒を促進することがわかり、同時にカルシウム沈着を主とする角膜混濁を抑制する働きがあることが証明され、角膜の透明維持機構の一部に迫るとともに角膜混濁を生じる疾患の治療への基盤となる知見となった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2016 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Remarks (2 results)

  • [Int'l Joint Research] Lancaster University(United Kingdom)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Development of functional human oral mucosal epithelial stem/progenitor cell sheets using a feeder-free and serum-free culture system for ocular surface reconstruction.2016

    • Author(s)
      Nakamura T, Yokoo S, Bentley AJ, Nagata M, Fullwood NJ, Inatomi T, Sotozono C, Yamagami S, Kinoshita S
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 37173-37173

    • DOI

      10.1038/srep37173

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Remarks] 京都府立医科大学眼科学教室 研究実績

    • URL

      http://www.ophth.kpu-m.ac.jp/actual_results-2/

    • Related Report
      2017 Research-status Report
  • [Remarks] Lancaster University, Research directory

    • URL

      http://www.research.lancs.ac.uk/portal/en/people/nigel-fullwood(07632a44-c3b1-408f-8753-a7e252e0f9c6).html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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