| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The purpose of this study is to examine the changes involved in the aging of cells in the cornea. Tissue stem cells from corneal layers were collected by sphere method. We examined the gene expression of stem cells and found that genes such as Nestin, which is a marker for undifferentiated nervous system, were highly expressed. Examination of the methylation of these cells demonstrated a high degree of methylation as compared to normal cultured cells. We could obtained daughter cells, which were high quality cells without shortening the telomere length or without staining with βGAL, from stem cells. We transplanted the regenerated cornea into an rabbit eyes. Transplanted cornea maintained transparency postoperatively. These results suggests that the transplanted undifferentiated cells may be a source of cell proliferation after transplantation.
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