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A trial in breast reconstruction surgery using platelet rich plasma and adipose derived stem cells

Research Project

Project/Area Number 16K11367
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plastic surgery
Research InstitutionTottori University

Principal Investigator

Yagi Shunjiro  鳥取大学, 医学部附属病院, 准教授 (00378192)

Co-Investigator(Kenkyū-buntansha) 福岡 晃平  鳥取大学, 医学部附属病院, 医員 (40548781)
久留 一郎  鳥取大学, 医学(系)研究科(研究院), 教授 (60211504)
陶山 淑子  鳥取大学, 医学部附属病院, 助教 (90448192)
森川 久未  鳥取大学, 医学部附属病院, 助教 (90707217)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords脂肪組織由来幹細胞(ADSCs) / 多血小板血漿(PRP) / 血管新生 / 細胞増殖能 / 分化誘導能 / 下肢虚血モデル / 血流改善 / 脂肪幹細胞 / 多血小板血漿 / 乳房再建 / 虚血 / 脂肪由来幹細胞 / 再生医療 / 移植 / 脂肪
Outline of Final Research Achievements

We had reported some positive results about using adipose derived stem cells(ADSCs) to breast deformation after breast-conserving surgery in clinical research. However, one problem is that atrophy of adipose tissues. To resolve this problem, we demonstrate the efficacy of adding platelet rich plasma(PRP), contains numerous growth factors useful for angiogenesis, cell proliferation and differentiation. As a results, the proliferation rate of ADSCs was higher in the optimum concentration of PRP, and we suggested that differentiation inducing ability from ADSCs to vascular endothelial cells at a specific concentration.
And that, we suggested that the ability of neovascuralization using a mouse ischemic hindlimb model according to laser Doppler perfusion scan. Against our expectation, blood flow ratios of ischemic limb to nomal limb were significantly higher in only ADSC group.

Academic Significance and Societal Importance of the Research Achievements

脂肪組織由来幹細胞(ADSCs)と多血小板血漿(PRP)の併用投与により,ADSCの細胞増殖能や血管内皮細胞への分化誘導能があることを証明できた.一方,下肢虚血モデルマウスでの1ヶ月間の血流改善度の追跡評価では,我々の予想に反し併用投与よりもADSCs単独投与で良好なデータが得られた.
In vitro実験は5日間と短期的な追跡であったが,局所投与によるvivoの実験は1ヶ月と比較的長期の追跡であったため,この間にPRPの局在性が失われてしまった可能性などが予想された.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017 2016

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Pretreatment with an angiotensin II receptor blocker abolished ameliorating actions of adipose-derived stem cell sheets on cardiac dysfunction and remodeling after myocardial infarction2018

    • Author(s)
      Yamamoto Kenshiro、Kurata Yasutaka、Inoue Yumiko、Adachi Maya、Tsuneto Motokazu、Miake Junichiro、Ogino Kazuhide、Ninomiya Haruaki、Yoshida Akio、Shirayoshi Yasuaki、Suyama Yoshiko、Yagi Shunjiro、Nishimura Motonobu、Yamamoto Kazuhiro、Hisatome Ichiro
    • Journal Title

      Regenerative Therapy

      Volume: 9 Pages: 79-88

    • DOI

      10.1016/j.reth.2018.08.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of postoperative doxorubicin administration on ischemic wound healing2018

    • Author(s)
      Morishita T, Toriyama K, Takanari K, Yagi S, Ebisawa K, Hishida M, Narita Y, Osaga S, Nishida Y, Kamei Y.
    • Journal Title

      Nagoya Journal of Medical Science

      Volume: 80 Issue: 3 Pages: 357-366

    • DOI

      10.18999/nagjms.80.3.357

    • NAID

      120006502469

    • ISSN
      2186-3326
    • URL

      https://nagoya.repo.nii.ac.jp/records/26326

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of Fat Grafts for Stabilizing Microvascular Pedicle Geometry in Lower Limb Reconstruction.2018

    • Author(s)
      Yagi S, Suyama Y, Yamaga K, Morita M, Fukuoka K.
    • Journal Title

      Plast Reconstr Surg Glob Open.

      Volume: 13 Issue: 7 Pages: e1799-e1799

    • DOI

      10.1097/gox.0000000000001799

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] フコイダン前投与により誘導される虚血肢の血管新生作用に関する研究2018

    • Author(s)
      野津智美, 櫻木 哲詩, 陶山 淑子, 八木 俊路朗, 久留 一郎
    • Journal Title

      米子医学雑誌

      Volume: 69 Pages: 16-24

    • NAID

      120006862011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Fat Grafts for Stabilizing Microvascular Pedicle Geometry in Head and Neck Reconstruction2017

    • Author(s)
      Shunjiro Yagi, Yoshiko Suyama, Kohei Fukuoka, Maki Morita, Miki Kambe, Kazuhiro Toriyama, Yuzuru Kamei
    • Journal Title

      Journal of Reconstructive Microsurgery Open

      Volume: 2 Issue: 02 Pages: e140-e144

    • DOI

      10.1055/s-0037-1608671

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 脂肪注入移植を用いた乳房再建術】 自己皮下脂肪組織由来幹細胞移植による乳房再建2016

    • Author(s)
      陶山淑子、福岡晃平、森川久未、久留一郎、八木俊路朗
    • Journal Title

      形成外科

      Volume: 59 Pages: 486-495

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 非培養皮下脂肪由来再生幹細胞(ADSCs)を用いた乳癌術後乳房変形に対する乳房再建の臨床試験2019

    • Author(s)
      陶山淑子、福岡晃平、森田真紀、八木俊路朗、久留一郎
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 自己皮下脂肪組識由来幹細胞(ADSC)による乳房再建術の長期経過2019

    • Author(s)
      陶山淑子、福岡晃平、森田真紀、八木俊路朗、久留一郎
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 遊離腓骨皮弁を用いた下顎再建において皮弁採取側を考慮することの重要性2018

    • Author(s)
      八木俊路朗、陶山淑子、福岡晃平、森田真紀、亀井譲
    • Organizer
      第36回日本頭蓋顎顔面外科学会
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2021-01-27  

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