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Treatment of old facial paralysis using layered adipose derived stem cell sheets

Research Project

Project/Area Number 16K11370
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plastic surgery
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Shimizu Yusuke  琉球大学, 医学(系)研究科(研究院), 教授 (10327570)

Co-Investigator(Kenkyū-buntansha) 角南 寛  琉球大学, 医学部, 特命助教 (50374723)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords脂肪組織由来幹細胞 / 顔面神経麻痺 / 細胞シート / 脂肪幹細胞 / 再生医療
Outline of Final Research Achievements

Under the approval of the Clinical Research Ethics Committee of the University of the Ryukyus (Approval No. 810), adipose tissue stem cells were extracted from human-derived adipose tissue, cultured for a few weeks with high purity, and a large amount of adipose stem cells were formed into collagen sheets. Seeding was performed to prepare a 4 cm 2 monolayer adipose stem cell sheet.Further, a layered sheet was produced by superposing the adipose derived stem cell sheets. Bilateral facial paralysis model rats were prepared, and the layered sheet was transplanted to each half of the face.On the side where the layered sheet was transplanted, facial nerve recovery was observed earlier than on the side where the laminated sheet was not transplanted.In addition, we developed a method for extracting adipose derived stem cells without using collagenase and filed a patent application.

Academic Significance and Societal Importance of the Research Achievements

陳旧性顔面神経完全麻痺、不全麻痺に伴う表情筋機能不全(萎縮、拘縮、病的共同運動)に有効な治療は確立されていない。顔面神経麻痺は笑えない、眼を閉じることができない等、罹患者の日常生活に多大な苦痛をもたらす疾患であり、日本でも数多くの患者が存在している。
本研究の結果、積層化した脂肪幹細胞シートを顔面神経麻痺患者さんに早期に移植することで、回復する可能性があることが示唆された。また本研究を通して、脂肪幹細胞シートを作製する技術を構築できた他、コラゲナーゼを用いず脂肪幹細胞を分離抽出する新しい方法を開発し特許出願を行えた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Advances in regenerative therapy: A review of the literature and future directions2020

    • Author(s)
      Ntege Edward H.、Sunami Hiroshi、Shimizu Yusuke
    • Journal Title

      Regenerative Therapy

      Volume: 14 Pages: 136-153

    • DOI

      10.1016/j.reth.2020.01.004

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 脂肪幹細胞の軟骨分化を促進する足場材の開発2019

    • Author(s)
      角南寛
    • Organizer
      第18回 日本再生医療学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 生分解性不織布を用いた再生治療用脂肪幹細胞シートの開発2018

    • Author(s)
      清水雄介
    • Organizer
      第27回 日本形成外科学会基礎学術集会 シンポジウム1
    • Related Report
      2018 Research-status Report
  • [Presentation] 培養脂肪幹細胞による陥凹性病変の治療2018

    • Author(s)
      清水雄介
    • Organizer
      第17回 日本再生医療学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 琉球大学における再生医療の現況と課題2018

    • Author(s)
      清水雄介
    • Organizer
      第132回 日本美容外科学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Clinical trial for the treatment of depressed facial lesions using cultured adipose derived stem cells2018

    • Author(s)
      Yusuke Shimizu
    • Organizer
      3rd International BioMedical Interface Symoposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 培養ヒト脂肪組織由来幹細胞を用いた臨床研究の開始 ~ゼロからのスタート~2016

    • Author(s)
      清水雄介
    • Organizer
      第59回日本形成外科学会総会・学術集会教育セミナー
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] コラゲナーゼを用いないで脂肪組織由来幹細胞を分離抽出するための方法、及び脂肪由来幹細胞分離抽出用キット2019

    • Inventor(s)
      清水雄介、角南寛、普天間寛子、オルソリバース株式会社
    • Industrial Property Rights Holder
      清水雄介、角南寛、普天間寛子、オルソリバース株式会社
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2018 Research-status Report
    • Overseas

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Published: 2016-04-21   Modified: 2021-02-19  

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