Promoter analysis of sweet and umami taste receptor genes
| Project/Area Number |
16K11455
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中富 満城 九州歯科大学, 歯学部, 講師 (10571771)
片岡 真司 九州歯科大学, 歯学部, 助教 (80364149)
瀬田 祐司 九州歯科大学, 歯学部, 教授 (90291616)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 転写 / Tas1r1 / Klf5 / プロモーター / うま味 / 転写因子 / 味覚受容体 / 転写調節 / KLF5 / アミノ酸 / T1R1 / T1R3 / 甘味受容体 / うま味受容体 / 転写制御 |
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| Outline of Final Research Achievements |
In this study, we examined the function of Tas1r1 promoter in the mouse C2C12 myoblasts. Reporter assays showed that a 148-bp region upstream of the start codon of Tas1r1 had a promoter activity. Site-directed mutagenesis of GT-box in this promoter significantly reduced the promoter activation. RNAi-mediated depletion of Sp4 and Klf5 decreased Tas1r1 expression. The overexpression of Klf5, but not Sp4, significantly increased Tas1r1 expression. The ENCODE data of chromatin immunoprecipitation and sequencing (ChIP-seq) showed that Klf5 bound to the GT-box. Furthermore, the Klf5 knockout cell lines led to a considerable decrease in the levels of Tas1r1 expression. Taken together, these results showed that Klf5 binds to the GT-box in the Tas1r1 promoter and regulates Tas1r1 expression in C2C12 cells.
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| Academic Significance and Societal Importance of the Research Achievements |
高齢者に多い感覚器障害の一つであり、味覚障害を予防、治療することは、高齢者におけるQOLの維持を考える上で重要であると考えられる。味覚受容体発現の低下が認められる味覚障害患者においては、甘味、うま味受容体T1Rファミリーの転写を活性化させる転写因子が、機能不全に陥っている可能性が考えられる。本研究により、うま味受容体Tas1r1遺伝子の転写の活性化に、転写因子Klf5が関与していることが明らかになった。転写の活性化には、複数の転写因子が関わっていることから、本研究を端緒として、他の転写因子を含めた転写活性化機構が明らかになれば、その機能不全のメカニズムの解明にもつながると考えられる。
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Anticancer effect of novel platinum nanocomposite beads on oral squamous cell carcinoma cells2019
Author(s)
Tanaka M., Okinaga T., Iwanaga K., Matsuo K., Toyono T., Sasaguri M., Ariyoshi W., Tominaga K., Enomoto Y., Matsumura Y., Nishihara, T.
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Journal Title
J Biomed Mater Res B Appl Biomater
Volume: 9999B
Issue: 7
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access
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