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Response of epithelial cells infected by Treponema denticola

Research Project

Project/Area Number 16K11461
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

Eitoyo Kokubu  東京歯科大学, 歯学部, 講師 (70453785)

Co-Investigator(Kenkyū-buntansha) 石原 和幸  東京歯科大学, 歯学部, 教授 (00212910)
菊池 有一郎  東京歯科大学, 歯学部, 講師 (30410418)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsTreponema denticola / 細胞侵入 / サイトカイン / 歯周病 / 免疫応答 / 口腔粘膜細胞 / TLR2 / dentilisin / 主要膜タンパク / 口腔粘膜上皮細胞 / 口腔細菌 / 細菌 / 歯学 / 微生物
Outline of Final Research Achievements

To clarify the pathogenicity of T. denticola, epithelial cells were infected with the wild-type strain or a mutant strain deficient in dentilisin or a major outer sheath protein (ΔprtP or Δmsp respectively). The migration activity of cells infected by ΔprtP compared with those infected by wild-type strain and Δmsp. TLR-2 signal intensity was greater in the cells infected by ΔprtP than in cells infected by the wild-type strain.
These results indicate that prtP modulates pathogen recognition via TLR2 and that dentilisin has the potential to degrade paxillin and HSP70, which decreases cell migration and delays stress response. Collectively, the findings show that prtP modulates the immune response of host cells to T. denticola infection.

Academic Significance and Societal Importance of the Research Achievements

本研究によりT. denticolaの病原性因子による口腔粘膜上皮組織への感染および細胞侵入のメカニズムが明らかになる。本菌の病原性の解明および宿主免疫に対する回避機構は、慢性歯周病疾患の機序の解明や口腔内細菌の侵入予防への応用の糸口となる。さらに本菌の構成成分であるMspは、梅毒の病原体であるTreponema pallidamとの遺伝学的に相同分子があることが知られているため、本研究の成果は、歯周病の病原性に限局されず、Treponema属全体の病原性メカニズムの解析につながり、梅毒あるいはDigital dermatitis等のTreponema感染症の治療に繋がる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Inhibitory Effects of Lingonberry Extract on Oral Streptococcal Biofilm Formation and Bioactivity2019

    • Author(s)
      Kokubu Eitoyo、Kinoshita Emiko、Ishihara Kazuyuki
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 60 Issue: 1 Pages: 1-9

    • DOI

      10.2209/tdcpublication.2018-0007

    • NAID

      130007605372

    • ISSN
      0040-8891
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <i>Treponema denticola</i> Induces Epithelial Barrier Dysfunction in Polarized Epithelial Cells2018

    • Author(s)
      Kikuchi Yuichiro、Kimizuka Ryuta、Kato Tetsuo、Okuda Katsuji、Kokubu Eitoyo、Ishihara Kazuyuki
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 59 Issue: 4 Pages: 265-275

    • DOI

      10.2209/tdcpublication.2017-0052

    • NAID

      130007538122

    • ISSN
      0040-8891
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Response of epithelial cells infected by Treponema denticola2018

    • Author(s)
      Kokubu E, Inoue T, Ishihara K.
    • Journal Title

      Oral disease

      Volume: 24 Issue: 1-2 Pages: 14-18

    • DOI

      10.1111/odi.12794

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 1.臨床と基礎の架け橋 Treponema denticolaの細胞侵入機構の解析2018

    • Author(s)
      国分 栄仁
    • Organizer
      第306回東京歯科大学学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 2.Treponema denticolaのprtPは上皮細胞の免疫応答を変調させる2018

    • Author(s)
      国分 栄仁, 菊池 有一郎, 柴山 和子, 石原 和幸
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Treponema denticolaの感染による細胞応答2018

    • Author(s)
      3.国分栄仁, 菊池有一郎, 柴山和子, 石原和幸
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Response of epithelial cells infected by Treponema denticola2018

    • Author(s)
      国分栄仁,菊池有一郎,柴山和子,石原和幸
    • Organizer
      第91回細菌学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Treponema denticolaの細胞侵入に影響する病原因子の解析2017

    • Author(s)
      国分 栄仁
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Remarks] 東京歯科大学微生物学講座 研究業績

    • URL

      http://portal.tdc.ac.jp/dept/mb/page1/page1.html

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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