Application of peptide (P11-4) to remineralization of acid tooth

• Project/Area Number: 16K11564
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: KUROKAWA Hiroyasu 日本大学, 歯学部, 准教授 (10291709)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 酸蝕歯 / 自己集合性ペプチドP11-4 / 脱灰抑制 / 再石灰化 / 自己集合性ペプチド / 弾性率 / ペプチドP11-4 / 酸蝕歯予防

Outline of Final Research Achievements

A new product containing the peptide P11-4 was recently introduced to enhance remineralization and inhibit demineralization of the tooth substrate. Peptide P11-4 self-assembles into a three-dimensional (3D) scaffold on the surface of the tooth, where it promotes hydroxyapatite precipitation while inhibiting diffusive mineral loss. Therefore, due to its mineralization effect, this material is potentially useful not only for early carious lesions but also for enamel defects caused by erosion and tooth wear. Considering the potential of the self-assembly peptide P11-4 to enhance enamel remineralization, the purpose of this study was to use ultrasonic measurements to evaluate the preventive effects of peptide P11-4 on acidic erosion of enamel substrates.
Within the limitations of this in vitro study, application of the self-assembling peptide P11-4 may be able to prevent the progress of erosion from acid challenge and even to promote remineralization.

Academic Significance and Societal Importance of the Research Achievements

酸蝕歯は食生活習慣の変化などによって顕著化した第3の硬組織疾患とされているが、その予防法については確立されていないのが現状である。ペプチドP11-4は、低いpH環境下でカルシウムイオンを吸着するための足場となることが判明しており、酸蝕が進行する口腔内環境でこそ、歯質の脱灰抑制、再石灰化効果を発揮する可能性がある。本研究の結果はこれを裏付けるものであり、ペプチドP11-4が酸蝕歯の予防に有効であることが示された。

Research Products

• [Journal Article] Application of the self-assembling peptide P11-4 for prevention of acidic erosion． (2018)
  • Author(s): Suda S, Takamizawa T, Takahashi F, Tsujimoto A, Akiba S, Nagura Y, Kurokawa H, Miyazaki M
  • Journal Title: Operative Dentistry Volume: - Issue: 4 Pages: E166-E172
  • DOI: 10.2341/17-175-l
  • Peer Reviewed
• [Journal Article] Ultrasonic assessment of the effects of self-assembling peptide scaffolds on preventing enamel demineralization (2016)
  • Author(s): Takahashi F, Kurokawa H, Shibasaki S, Kawamoto R, Murayama R, Miyazaki M
  • Journal Title: Acta Odontol Scand Volume: 74 Issue: 2 Pages: 142-147
  • DOI: 10.3109/00016357.2015.1066850
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ペプチドP11-4を応用した酸蝕歯の戦略的予防法の確立 (2018)
  • Author(s): 須田駿一，黒川弘康，髙見澤俊樹，石井 亮，飯野正義，宮崎真至，升谷滋行
  • Organizer: 第70回日本大学歯学会学術大会
• [Presentation] ペプチドP11-4の応用による酸蝕歯の再石灰化効果 (2018)
  • Author(s): 須田駿一，黒川弘康，古市哲也，関東英貴，杉村留奈，宮崎真至，若松英輝
  • Organizer: 日本歯科保存学会2018年度春季学術大会（第148回）
• [Presentation] Application of the self-assembling peptide P11-4 on acid erosion prevention (2017)
  • Author(s): Takahashi F, Suda S, Keiichi Sai, Kurokawa H, Hinoura K, Miyazaki M
  • Organizer: 95TH General Session & Exhibition of the IADR
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 酸蝕歯予防への自己集合性ペプチドP11-4の応用 (2016)
  • Author(s): 須田駿一，黒川弘康，高橋史典，松吉佐季，佐藤愛子，村山良介，宮崎真至，鈴木敏裕
  • Organizer: 日本歯科保存学会2016年度秋季学術大会（第145回）
  • Place of Presentation: キッセイ文化ホール（長野県松本市水汲）
  • Year and Date: 2016-10-28