Development of alveolar bone augmentation by nerve guidance

• Project/Area Number: 16K11604
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Kagoshima University
• Principal Investigator: Suehiro Fumio 鹿児島大学, 医歯学域附属病院, 講師 (40524781)
• Co-Investigator(Kenkyū-buntansha): 西村 正宏 鹿児島大学, 医歯学域歯学系, 教授 (00294570) ; 石井 正和 鹿児島大学, 医歯学域歯学系, 助教 (00456683)
• Research Collaborator: Komabashiri Naohiro
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨髄由来間質細胞 / 顎骨骨髄 / 顎骨再生 / BDNF / 顎骨骨髄間質細胞 / 顎骨増生 / 神経誘導

Outline of Final Research Achievements

Maxillary/Mandibular Bone Marrow Stromal Cells (MBMSC) express TrkB, which is a receptor for brain-derived neurotrophic factor (BDNF), and it is suggested that BDNF is secreted in the culture supernatant. Cell proliferation ability and bone differentiation ability of iliac bone marrow-derived mesenchymal stem cells were found to be not affected by BDNF in vitro, but further investigations on the influence of BDNF on bone differentiation of MBMSC is necessary.

Academic Significance and Societal Importance of the Research Achievements

近年、再生医療に関する研究は飛躍的な発展を遂げており、再生医療等製品の承認も進んでいる。しかしながら、いまだ骨再生を目的とした再生医療等製品の承認はなされていない。本研究結果が骨再生医療に直結するわけではないが、骨再生のメカニズムを解析することで骨形成を促進させることが出来れば、我々の目指す顎骨再生医療だけでなく、医科領域における骨粗鬆症予防や骨折治癒薬開発への波及効果も期待される。

Research Products

• [Journal Article] Vascular Endothelial Growth Factor-C Induces Osteogenic Differentiation of Human Mesenchymal Stem Cells through the ERK and RUNX2 Pathway (2017)
  • Author(s): Murakami J, Ishii M, Suehiro F, Ishihata k, Nakamura N, Nishimura M
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 484 Issue: 3 Pages: 710-718
  • DOI: 10.1016/j.bbrc.2017.02.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Low-serum culture with novel medium promotes maxillary/mandibular bone marrow stromal cell proliferation and osteogenic differentiation ability (2017)
  • Author(s): Suehiro F, Ishii M, Asahina I, Murata H, Nishimura M
  • Journal Title: Clinical Oral Investigation Volume: 印刷中 Issue: 9 Pages: 2709-2719
  • DOI: 10.1007/s00784-017-2073-7
  • Peer Reviewed
• [Journal Article] Diversity of multinucleated giant cells by microstructures of hydroxyapatite and plasma components in extraskeletal implantation model (2016)
  • Author(s): Morishita K, Tatsukawa E, Shibata Y, Suehiro F, Kamitakahara M, Yokoi T, Ioku K, Ueda M, Nishimura M, Ikeda T
  • Journal Title: Acta Biomaterialia Volume: 39 Pages: 180-191
  • DOI: 10.1016/j.actbio.2016.05.002
  • Peer Reviewed
• [Journal Article] Regulation and Biological Significance of Formation of Osteoclasts and Foreign Body Giant Cells in an Extraskeletal Implantation Model (2016)
  • Author(s): Ahmed GJ, Tatsukawa E, Morishita K, Shibata Y, Suehiro F, Kamitakahara M, Yokoi T, Koji T, Umeda M, Nishimura M, Ikeda T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 49 Issue: 3 Pages: 97-107
  • DOI: 10.1267/ahc.16007
  • NAID: 130005161006
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 顎骨骨髄由来間質細胞の骨形成能判定のためのマーカー探索 (2019)
  • Author(s): 駒走尚大，末廣史雄，石井正和，柳澤嵩大，原田佳枝，西村正宏
  • Organizer: 第18回日本再生医療学会総会
• [Presentation] 顎堤増生治療を目的とした顎骨骨髄由来間質細胞の採取成績 (2019)
  • Author(s): 末廣史雄, 益﨑与泰, 田中謙光, 駒走尚大, 西村正宏
  • Organizer: 第36回日本口腔インプラント学会九州支部学術大会
• [Presentation] 顎骨骨髄由来間質細胞を用いた骨増生法の開発 (2018)
  • Author(s): 末廣史雄
  • Organizer: 平成30年度日本補綴歯科学会九州支部会
  • Invited
• [Presentation] 顎骨再生を目的とした顎骨骨髄間質細胞培養法の開発 (2017)
  • Author(s): 末廣史雄，藤島 慶，益﨑与泰，原田佳枝，柳澤嵩大，西村正宏
  • Organizer: 第126回日本補綴歯科学会
• [Presentation] 吸収性乳酸/グリコール酸共重合体（PLGA）膜と非吸収性高密度4フッ化エチレン樹脂（d-PTFE）膜を用いたGBRの比較検討 (2017)
  • Author(s): 末廣史雄，益﨑与泰，石畑清秀，村上寿理，中村利明，西村正宏
  • Organizer: 第47回日本口腔インプラント学会
• [Presentation] 顎骨増生を目的とした低侵襲・効率的な顎骨骨髄間質細胞培養法の開発 (2016)
  • Author(s): 末廣史雄，益﨑与泰，原田佳枝，柳澤嵩大，西村正宏
  • Organizer: 平成28年度日本補綴歯科学会九州支部，中国・四国支部合同学術大会
  • Place of Presentation: 熊本県歯科医師会館（熊本県・熊本市）
  • Year and Date: 2016-09-04
• [Presentation] Is Cellular Distribution Crucial Factor (2016)
  • Author(s): Sakai Y, Nishimura M, Suehiro F, Shigemitsu Y, Funabashi T, Kumagai T
  • Organizer: 94th General Session & Exhibition of the IADR
  • Place of Presentation: Seoul Korea
  • Year and Date: 2016-06-24
  • Int'l Joint Research