• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation on oxidative stress and damage of body-defense-cells caused by monomers and consideration of protective methods

Research Project

Project/Area Number 16K11630
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Prosthodontics/ Dental materials science and
Research InstitutionIwate Medical University

Principal Investigator

TAIRA MASAYUKI  岩手医科大学, 歯学部, 准教授 (60179398)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords歯科用モノマー / トリエチレングリコールジメタクリレート / 単球 / グルタチオン / 解毒 / 抗酸化 / 炎症性サイトカイン / LPS / THP-1 / マクロファージ / 安全性評価 / 酸化ストレス / 活性酸素種 / 単球細胞 / フォルボールエステル / 解毒化酵素 / 歯学 / モノマー / シグナル伝達 / ストレス / 生理活性
Outline of Final Research Achievements

Monomers in dental resins irritate oral tissues. The purpose of this study was to evaluate effects of IC50 tri-ethelenglycol di-metahcrylate (TEGDMA) on gene expressions of lipopolysaccharide (LPS)-stimulated monocytic THP-1 cells. We compared gene expressions of two samples, namely (i) THP-1 cells stimulated with LPS for 4h (Control) and (ii) those exposed to IC50 TEGDMA for 24h and LPS for last 4h (Test) by DNA microarray analyses. Thirty four genes of Test were up-regulated more than 5-fold, relative to those of Control (p<0.05). The up-regulated genes of Test had two characteristic biological processes, namely xenobiotic metabolism (e.g. AKR1C1 and GSTA4 genes) and anti-oxidative stress (e.g. NQO1, GPX3, and SELM genes). These gene expressions were confirmed by quantitative real-time RT-PCR analyses.

Academic Significance and Societal Importance of the Research Achievements

TEGDMAモノマー単独作用下およびLPSとモノマーの二重作用下での単球(THP-1)の細胞内傷害作用(酸化ストレス傷害、炎症性サイトカイン傷害、モノマー分解産物傷害)を遺伝子発現から明らかにしたことは学術的に意義があると考えられた。TEGDMAモノマーによる単球の細胞内障害を還元型グルタチオンの薬物代謝と枯渇による酸化ストレスとの関係で考察する試みは有益と思われた。モノマーによる口腔組織への為害性の減少については、細胞内でグルタチオンを補充することが有効と考えられ、そのための薬物投与法の開発が期待された。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Ectopic bone formation in muscles using injectable bone-forming material consisting of cross-linked hyaluronic acid, bone morphogenetic protein, and nano-hydroxyapatite2019

    • Author(s)
      Ikeda K, Sugawara S, Taira M, Sato H, Hatakeyama W, Kihara H, Kondo H.
    • Journal Title

      Nano Biomedicine

      Volume: 11 Pages: 11-20

    • NAID

      130007687555

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loading of fluvastatin onto gelatin-coated titanium implants.2018

    • Author(s)
      Takemoto S, Sasaki K, Sugawara S, Saitoh S, Sawada T, Taira M, Tanabe K, Yoshinari M, Hattori M, Jansen JA, Leeuwenburgh SGG.
    • Journal Title

      Key Engineering Materials

      Volume: 782 Pages: 233-237

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bone marrow-derived mesenchymal stem cells propagate immunosuppressive/anti-inflammatory macrophages in cell-to-cell contact-independent and -dependent manners under hypoxic culture2017

    • Author(s)
      Takizawa N, Okubo N, Kamo M, Chosa N, Mikami T, Suzuki K, Yokota S, Ibi M, Ohtsuka M, Taira M, Sasaki D, Yaegashi T, Ishisaki A, Kyakumoto S
    • Journal Title

      Experimental Cell Res.

      Volume: 358 Issue: 2 Pages: 411-420

    • DOI

      10.1016/j.yexcr.2017.07.014

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bone regeneration of rat critical-size calvarial defects using a collagen/porous-apatite composite: Micro-CT analyses and histological observations2017

    • Author(s)
      Hatakeyama W, Taira M, Ikeda K, Sato H, Kihara H, Tekemoto S, Kondo H.
    • Journal Title

      J Oral Tissue Engin.

      Volume: 15 Pages: 49-60

    • NAID

      130006394589

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of addition of nano-hydroxyapatite to highly-pressed collagen on osteogenic differentiation in osteoblastic SaOS-2 cells.2016

    • Author(s)
      Ikeda K., Taira M, Yokota J, Hattori M, Ishisaki A, Kondo H.
    • Journal Title

      Nano Biomedicine

      Volume: 8 Pages: 91-100

    • NAID

      130005243671

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] スタチン系薬剤を固定したチタンの薬剤徐放特性2019

    • Author(s)
      武本 真治,澤田 智史,畑中 昭彦,平 雅之
    • Organizer
      第49回日本口腔インプラント学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ろう付けしたステンレス鋼の酸性溶液中での溶出挙動2019

    • Author(s)
      齋藤設雄,佐々木かおり,澤田智史,畑中昭彦,平 雅之,武本真治
    • Organizer
      74回日本歯科理工学会学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 重度インプラント周囲炎に対し外科的デブライトメントと自家骨移植を併用した症例2018

    • Author(s)
      菅原志帆,畠山 航,高藤恭子,武本真治,平 雅之,鬼原英道,西郷 慶悦,近藤尚知.
    • Organizer
      第48回日本口腔インプラント学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Mesenchymal stem cells educate undifferentiated monocyte/macrophages to the M2 macrophages in cell-cell adhesion-dependent and independent ways2017

    • Author(s)
      Takizawa N, Okubo N, Kamo M, Chosa N, Mikami T, Suzuki K, Yokota S, Ibi M, Ohtsuka M, Taira M, Sasaki D, Yaegashi T, Ishisaki A, Kyakumoto S
    • Organizer
      第65回国際歯科研究学会日本部会(JADR)学術大会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 架橋型ヒアルロン酸・ナノアパタイト・BMPを用いた注入式骨補填材の試作2016

    • Author(s)
      池田功司,平 雅之,畠山 航,高藤恭子,近藤尚知.
    • Organizer
      第46回日本口腔インプラント学会
    • Place of Presentation
      名古屋市
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi