| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The in vitro embryotoxicities of metals (Ti, V, Al, Nb, Ta, and Zr) used in a commercially available implant made of titanium alloy were examined by the EST protocol, demonstrating no embryotoxicity for any metal, except vanadium. The survival rates of mouse 3T3, ES-D3, and iPS cells based on the MTT method were lower in the fluoride-treated group than in the negative controls (i.e., untreated ground powder and plate made of titanium alloy).
In addition, the test solution, prepared by immersing corroded, ground powder (hydrofluoric acid-treated ground powder of Ti-6Al-4V plate (ASTM F136)) into a cell culture medium, reduced the rates of contraction in teratomas, due to differentiation, in both ES-D3 and iPS cells, as compared with untreated titanium alloy. However, the ALP activities showed no significant difference. No embryotoxicity was demonstrated, even though the experimental conditions were more severe than the conditions on actual use.
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