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the periodontal ligament-derived cell sheet cultured on amniotic membrane substrate for bone regeneration and tissue repair.

Research Project

Project/Area Number 16K11695
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Amemiya Takeshi  京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (90398389)

Co-Investigator(Kenkyū-buntansha) 足立 哲也  京都府立医科大学, 医学(系)研究科(研究院), 助教 (10613573)
山本 俊郎  京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472)
金村 成智  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70204542)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords羊膜 / 骨再生 / IGF-1 / SDF-1 / 歯学 / 移植・再生医療 / 再生医学 / 細胞・組織 / 臨床
Outline of Final Research Achievements

The purpose of this study is to develop a new cultured cell sheet effective for periodontal tissue regeneration. Amniotic membrane is a biomaterial that has anti-inflammatory and anti-infective properties and its attention as a useful and effective substrate for various cells. We have previously prepared cultured human periodontal ligament fibroblast (hPdLF) sheets using amniotic membrane as a substrate and reported that they have the ability to regenerate new bone (periodontal tissue) when transplanted into experimental animals. We also found that the use of amniotic membrane as the culture substrate for hPdLF promoted the production of chemokines SDF-1 / CXCL12 and CXCL14, and found the usefulness of amniotic membrane.

Academic Significance and Societal Importance of the Research Achievements

羊膜上培養PDL細胞シートは骨分化能だけでなく,成長因子IGF-1やケモカインSDF-1/CXCL12やCXCL14の産生が促進され,羊膜の有用性を見出した.羊膜を基板とした細胞移植は,新たな骨再生医療への応用の可能性が示された。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016

All Journal Article (6 results) (of which Open Access: 2 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 9 results)

  • [Journal Article] Transplantation of dental pulp‐derived cell sheets cultured on human amniotic membrane induced to differentiate into bone2019

    • Author(s)
      Takizawa Shigeta、Yamamoto Toshiro、Honjo Ken‐Ichi、Sato Yoshiki、Nakamura Koya、Yamamoto Kenta、Adachi Tetsuya、Uenishi Toshihiro、Oseko Fumishige、Amemiya Takeshi、Yamamoto Yoshiaki、Kumagai Wataru、Kita Masakazu、Kanamura Narisato
    • Journal Title

      Oral Diseases

      Volume: 25 Issue: 5 Pages: 1352-1362

    • DOI

      10.1111/odi.13096

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 羊膜上培養自己口腔粘膜上皮細胞シートによる口腔粘膜の再建2018

    • Author(s)
      雨宮 傑,山本俊郎,金村成智
    • Journal Title

      日本歯科評論

      Volume: 78(7) Pages: 155-158

    • Related Report
      2018 Research-status Report
  • [Journal Article] 羊膜を用いた培養歯根膜細胞シートによる歯周組織の再生2018

    • Author(s)
      雨宮 傑,山本俊郎,金村成智
    • Journal Title

      日本歯科評論

      Volume: 78(8) Pages: 159-162

    • Related Report
      2018 Research-status Report
  • [Journal Article] 培養細胞シートを用いた新規骨再生医療の開発2018

    • Author(s)
      雨宮 傑,山本俊郎,金村成智
    • Journal Title

      日本歯科評論

      Volume: 78(9) Pages: 155-158

    • Related Report
      2018 Research-status Report
  • [Journal Article] Development of Cell Sheets Cultured on Amniotic Membrane Aiming at Periodontal Tissue Regeneration2017

    • Author(s)
      雨宮 傑
    • Journal Title

      The Japanese Journal of Conservative Dentistry

      Volume: 60 Issue: 6 Pages: 270-272

    • DOI

      10.11471/shikahozon.60.270

    • NAID

      130006304467

    • ISSN
      0387-2343, 2188-0808
    • Related Report
      2017 Research-status Report
    • Open Access
  • [Journal Article] Development of Human Periosteal-derived Cell Sheets Cultured on an Amniotic Membrane Substrate2016

    • Author(s)
      Amemiya T, Endo Y, Asai T, Adachi T, Adachi K, Nishigaki M, Oseko F, Yamamoto T, Kanamura N
    • Journal Title

      The Japanese Journal of Conservative Dentistry

      Volume: 59 Issue: 5 Pages: 394-401

    • DOI

      10.11471/shikahozon.59.394

    • NAID

      130005277316

    • ISSN
      0387-2343, 2188-0808
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Effect of amniotic membrane for periodontal ligament fibroblast cell sheet.2018

    • Author(s)
      Amemiya T, Adachi T, Adachi K, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      96th General Session & Exhibition of the International Association for Dental Research (IADR)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of amniotic membrane as a substrate for human periodontal ligament fibroblast cell sheet on growth factor production2018

    • Author(s)
      Amemiya T, Adachi T, Adachi K, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      The 5th World Congress of the Tissue Engineering and Regenerative Medicine International Society - 2018 Kyoto, Japan (TERMIS-WC 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト羊膜上培養歯髄由来細胞シートを用いた歯槽骨再生の評価2018

    • Author(s)
      山野辺広中、山本俊郎、堀口智史、中井 敬、山本健太、大迫文重、雨宮 傑、坂下敦宏、中村 亨、金村成智
    • Organizer
      日本歯科保存学会2018年度秋季学術大会(第149回)
    • Related Report
      2018 Research-status Report
  • [Presentation] Human Periosteal-derived Cell Sheets Cultured on Amniotic Membrane Substrate.2017

    • Author(s)
      Amemiya T, Adachi T, Asai T, Adachi K, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      95th General Session & Exhibition of the International Association for Dental Research (IADR)
    • Place of Presentation
      San Francisco, Calif., USA
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 羊膜基質がヒト歯根膜線維芽細胞シートの成長因子産生に与える影響について2017

    • Author(s)
      雨宮 傑,足立哲也,足立圭司,大迫文重,山本俊郎,金村成智
    • Organizer
      第70 回 NPO法人日本口腔科学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Search for genes involved in periodontal tissue regeneration expressed by periodontal ligament cell sheets cultured on amniotic membrane substrate.2017

    • Author(s)
      Adachi K, Amemiya T, Adachi T, Nishigaki M, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      39th Asia Pacific Dental Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cultivate of periosteal-derived cell sheets on an amniotic membrane substrate.2017

    • Author(s)
      Amemiya T, Adachi T, Adachi K, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      39th Asia Pacific Dental Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bone differentiation induction of dental pulp-derived cell sheet cultured on human amniotic membrane and its application to alveolar bone regeneration.2017

    • Author(s)
      Yamamoto T, Honjo K, Amemiya T, Asai T, Kita M, Kanamura N.
    • Organizer
      39th Asia Pacific Dental Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト歯根膜細胞シートの成長因子産生に羊膜が及ぼす影響について2017

    • Author(s)
      雨宮 傑,足立哲也,遠藤悠美,市岡宏顕,足立圭司,大迫文重,山本俊郎,金村成智
    • Organizer
      日本歯科保存学会2017年度春季学術大会(第146回)
    • Related Report
      2017 Research-status Report
  • [Presentation] Examination of implanted dental pulp-derived cell sheets cultured on amnion.2016

    • Author(s)
      Yamamoto T, Honjo K, Nishigaki M, Amemiya T, Kita M, Kanamura N.
    • Organizer
      94th General Session and Exhibition of the International Association of Dental Research (IADR)
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Immunohistochemical investigation of periosteal-derived cell sheet cultured on amnion.2016

    • Author(s)
      Amemiya T, Adachi T, Adachi K, Nishigaki M, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      38th Asia Pacific Dental Congress
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Examination of osteogenic potential in nude mice transplantation of the periodontal ligament-derived cell sheet cultured on amnion.2016

    • Author(s)
      Adachi K, Amemiya T, Adachi T, Nishigaki M, Oseko F, Yamamoto T, Kanamura N.
    • Organizer
      38th Asia Pacific Dental Congress
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 羊膜を培養基質とした培養歯根膜由来細胞シートにおける歯周組織再生に関わる因子の検索2016

    • Author(s)
      雨宮 傑,足立哲也,遠藤悠美,市岡宏顕,足立圭司,西垣 勝,大迫文重,山本俊郎,金村成智
    • Organizer
      日本歯科保存学会2016年度春季学術大会(第144回)
    • Place of Presentation
      栃木県総合文化センター(栃木)
    • Year and Date
      2016-06-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 膜上培養歯根膜由来細胞シートの組織修復ならびに骨再生に関与する遺伝子の検索2016

    • Author(s)
      雨宮 傑,足立哲也,市岡宏顕,足立圭司,西垣 勝,大迫文重,山本俊郎,金村成智
    • Organizer
      第70 回 NPO法人日本口腔科学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2016-04-17
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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