PD-1 and PD-L1 expression in oral squamous cell carcinoma

• Project/Area Number: 16K11697
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Wakayama Medical University
• Principal Investigator: Tojyo Itaru 和歌山県立医科大学, 医学部, 講師 (70405439)
• Co-Investigator(Kenkyū-buntansha): 平石 幸裕 和歌山県立医科大学, 医学部, 博士研究員 (40573449)
• Research Collaborator: Nakanishi Takashi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 口腔扁平上皮癌 / PD-L1 / p53 / CK17 / 免疫チェックポイント阻害剤 / 免疫チェックポイント阻害薬 / PD-1 / 免疫染色 / 細胞培養 / がん免疫療法

Outline of Final Research Achievements

We studied the correlation among immunohistochemical expressions of p53, CK17, PD-L1 in 48 biopsy samples of oral squamous cell carcinoma. Positive rate of p53, CK17, PD-L1(tumor cells) and PD-L1(infiltrating lymphocytes in tumor) is 63.2%, 91.7%, 48.9% and 57.1% respectively. Regarding tumor cells The expression of PD-L1 and p53 positively correlated. Because resolution time of variant p53 protein delays and the protein is accumulated into a nucleus, the variant p53 protein is identified as overexpression. Although p53 inhibits the expression of PD-L1 directly, when variant p53 which lost a function is accumulated, PD-L1 overexpresses. Thus, it is thought that the expression of p53 and PD-L1 shows positively correlation in oral squamous cell carcinoma.

Academic Significance and Societal Importance of the Research Achievements

学術的には口腔扁平上皮癌において、p53の発現がPD-L1の発現に正の相関を示すことは、これまで報告されたことはなく、新知見である。社会的にはPD-L1の口腔扁平上皮癌組織における発現は、ニボルマブが口腔扁平上皮癌に奏功するかどうかのバイオマーカーの一つと考えられており、口腔扁平上皮癌においてp53がPD-L1の発現に正の相関を示すことが確認されれば、口腔扁平上皮癌組織におけるp53の発現もニボルマブが患者に奏功するかどうかのバイオマーカーの一つとして考えられる可能性がある。

Research Products

• [Journal Article] Comparison of Subjective and Objective Assessments of Neurosensory Function after Lingual Nerve Repair (2019)
  • Author(s): Shintani Yukari, Nakanishi Takashi, Ueda Masamichi, Mizobata Naoki, Tojyo Itaru, Fujita Shigeyuki
  • Journal Title: Medical Principles and Practice Volume: 2 Issue: 3 Pages: 1-5
  • DOI: 10.1159/000497610
  • Peer Reviewed / Open Access
• [Journal Article] Medication-related osteonecrosis of the jaw after tooth extraction in cancer patients: a multicenter retrospective study (2019)
  • Author(s): Hasegawa T, Hayashida S, Kondo E, Takeda Y, Miyamoto H, Kawaoka Y, Ueda N, Iwata E, Nakahara H, Kobayashi M, Soutome S, Yamada SI, Tojyo I, Kojima Y, Umeda M, Fujita S, Kurita H, Shibuya Y, Kirita T, Komori T
  • Journal Title: Osteoporosis International Volume: 30 Issue: 1 Pages: 231-239
  • DOI: 10.1007/s00198-018-4746-8
  • Peer Reviewed / Open Access
• [Presentation] 口腔扁平上皮癌におけるCK17とp53の発現とPD-L1の発現に関する臨床的および免疫組織学的検討 (2019)
  • Author(s): 東條 格、新谷ゆかり、中西 隆、岡本健二朗、平石幸裕、藤田茂之、惠中まゆ、佐藤冬樹、村垣泰光
  • Organizer: 第37回日本口腔腫瘍学会総会・学術大会
• [Presentation] 癌患者の口腔機能管理の基準に関する多施設共同後ろ向き観察研究 (2018)
  • Author(s): 山田 慎一、五月女 さき子、長谷川 巧実、東條 格、中原 寛和、河上 真緒、廣瀬 満理奈、藤田 茂之、古森 孝英、桐田 忠昭、渋谷 恭之、梅田 正博、栗田 浩
  • Organizer: 第56回日本癌治療学会学術集会
• [Presentation] Iatrogenic lingual nerve injuries : Retrospective study of 82 cases (2017)
  • Author(s): Itaru Tojo, Shigeyuki Fujita
  • Organizer: 7th Annual conference of Nepalese Association of Oral and Maxillofacial Surgeons (NAOMS) and Nepal-Japan Joint Scientific Meeting of Oral and Maxillofacial Surgeons
  • Place of Presentation: Kathmandu, Nepal
  • Int'l Joint Research