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Investigation of development and progression mechanism of oral candidiasis

Research Project

Project/Area Number 16K11702
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionTokyo Dental College

Principal Investigator

Shibayama Kazuko  東京歯科大学, 歯学部, 講師 (60408317)

Co-Investigator(Kenkyū-buntansha) 石原 和幸  東京歯科大学, 歯学部, 教授 (00212910)
柴原 孝彦  東京歯科大学, 歯学部, 教授 (50178919)
Project Period (FY) 2016-10-21 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsCandida albicans / 口腔カンジダ症 / 病原性 / バイオフィルム / カンジダ症 / バイオフィルム形成
Outline of Final Research Achievements

Surface antigen protein 2 (Csa2) is a member of the Candida albicans Common in Fungal Extracellular Membranes protein superfamily and we established its role in iron acquisition in this organism. To clarify further roles of Csa2, we compared growth, morphological transition, and biofilm formation among wild-type, Csa2-mutant, and complemented strains of C. albicans. Deletion of the Csa2 gene resulted in smaller and reduced colony growth, significant attenuation of the dimorphic transition under serum-inducing conditions, and reduced biofilm formation; complementation restored these levels to those of the wild-type. Our findings demonstrated that Csa2 participated in yeast-to-hyphae morphological switching under serum-inducing conditions and contributed to the biofilm formation of C. albicans. This work provided novel insights into the potential roles of Csa2 in virulence of C. albicans.

Academic Significance and Societal Importance of the Research Achievements

カンジダ・アルビカンスは多くの健常者より検出される常在性真菌であるが、防御機構に異常をもつ宿主に対しては真菌症を引き起こし、口腔咽頭カンジダ症を含む重篤な症状を呈する。本菌は、環境に適応して菌糸形になり菌糸を伸ばすことによって強い病原性を発揮し、粘膜下組織まで達し重篤化する。研究で明らかにした本菌の生体内増殖機構は、真菌の病原性の包括的理解と予防法および新規治療法確立への新たな基盤となるものである。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Characterization of a novel potential peptide import system in Treponema denticola2018

    • Author(s)
      Asai Tomohiro、Okamoto-Shibayama Kazuko、Kikuchi Yuichiro、Ishihara Kazuyuki
    • Journal Title

      Microbial Pathogenesis

      Volume: 123 Pages: 467-472

    • DOI

      10.1016/j.micpath.2018.07.045

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Possible Involvement of Surface Antigen Protein 2 in the Morphological Transition and Biofilm Formation of <I>Candida albicans</I>2017

    • Author(s)
      Okamoto-Shibayama Kazuko、Kikuchi Yuichiro、Kokubu Eitoyo、Ishihara Kazuyuki
    • Journal Title

      Medical Mycology Journal

      Volume: 58 Issue: 4 Pages: E139-E143

    • DOI

      10.3314/mmj.17-00008

    • NAID

      130006233737

    • ISSN
      2185-6486, 2186-165X
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Regulation of Gene Expression by marR-like Gene in Treponema denticola2017

    • Author(s)
      Y. Numata, K. Okamoto-Shibayama, T. Sato, Y. Miyai-Murai, Y. Kikuchi, E. Kokubu, K. Ishihara
    • Organizer
      95th General Session and Exhibition of the IADR
    • Place of Presentation
      San Francisco
    • Year and Date
      2017-03-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Isolation and Characterization of Dentipain in Treponema denticola2017

    • Author(s)
      Y. Miyai, K. Okamoto-Shibayama, T. Sato, Y. Numata, K. Ishihara.
    • Organizer
      95th General Session and Exhibition of the IADR
    • Place of Presentation
      San Francisco
    • Year and Date
      2017-03-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Treponema denticolaの細胞侵入に影響する病原因子の解析2017

    • Author(s)
      国分 栄仁,菊池 有一郎,柴山 和子,石原 和幸
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台市
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Porphyromonas gingivalisの鉄獲得機構におけるECFシグマ因子PGN_0319 の役割2017

    • Author(s)
      菊池 有一郎,太田 功貴,柴山 和子,国分 栄仁,齋藤 淳,石原 和幸
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台市
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Remarks]

    • URL

      http://www.tdc.ac.jp/dept/mb/page1/page1.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-10-24   Modified: 2021-02-19  

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