| Project/Area Number |
16K11751
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宮本 洋二 徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (20200214)
玉谷 哲也 徳島大学, 病院, 講師 (30274236)
高丸 菜都美 徳島大学, 大学院医歯薬学研究部(歯学域), 徳島大学専門研究員 (40513031)
永井 宏和 東北大学, 歯学研究科, 准教授 (50282190)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 唾液腺 / 再生 / マイクロアレイ解析 / miRNA / 遺伝子 / 顎下腺結紮モデルマウス / 腺房細胞 / 導管細胞 / iPS細胞 / 再生医療 |
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| Outline of Final Research Achievements |
The submandibular duct of C57BL/6JJcl mice were ligated with titanium clips. The microarray analysis showed that the expressions of 103 genes and 15 miRNAs of duct ligation group were increased 4 times more than those of non-duct ligation group. The target genes of candidate miR-710 were Clip 4 and Ier 5 (cut-off value : 2.5 fold change). The expression of Ier 5 was localized in not duct cells but acinar cells. And these were increased the 24 hours ligation as compared with untreatment.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究結果より，Clip 4やIer 5は唾液腺の萎縮に関与している可能性が示唆された。この成果は唾液腺組織再生の研究にも貢献できると考える。さらに、臨床研究へ誘導できる重要な基礎研究として位置づけられ、“唾液分泌低下の改善”という患者のQOL向上が期待できるだけでなく、唾液腺疾患への治療応用へも可能性を秘めていると考える。
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