Investigation of the individual difference of ability to salivate and the genetic factor for susceptibility to dental caries using chromosome substituted mice strains

• Project/Area Number: 16K11814
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: SHIMIZU Kunihiko 日本大学, 松戸歯学部, 准教授 (30328760)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 齲蝕感受性 / 遺伝因子 / 染色体置換マウス / 唾液分泌 / 唾液分泌量 / マウス第２番染色体 / コンジェニックマウス / 刺激時唾液分泌量 / 第2番染色体 / コンソミックマウス / 齲蝕症

Outline of Final Research Achievements

For investigate the genetic factors influenced to dental caries (tooth cavity) susceptibility, we established three congenic mice strain using consomic mice obtained with crossing inbred mice which property were high and low susceptibility of dental caries. From analytical results of the caries scores, the volume of stimulated saliva secretion and enamel hardness using 3 congenic strain mice, we suggested that salivary secretion volume was one of the most important factors associated with dental caries susceptibility in our experimental system, and the candidate genes for salivary secretion located between 84Mbp to 163Mbp on mice chromosome 2.

Academic Significance and Societal Importance of the Research Achievements

本研究では、虫歯になりやすいマウスと、なりにくいマウスを利用し、これら実験用マウスの虫歯になりにくい体質の１つとして、唾液分泌量の関与を直接的に証明した。さらに唾液分泌量に影響する遺伝因子が、マウス第２番染色体の一定領域に存在することを明らかにした。この領域はヒト第15番染色体に相当する。今後、この領域に存在し，虫歯の感受性に関与する遺伝子が特定できれば、ヒトにおいて、新しい虫歯予防法の開発に繋がると考えられる。

Research Products

• [Journal Article] Investigation of the effects of the chromosomal regions of mouse chromosome 2 on susceptibility to dental caries using congenic strains (2019)
  • Author(s): Watanabe Chihiro、Orino Daisuke、Shimizu Kunihiko
  • Journal Title: Pediatric Dental Journal Volume: 29 Issue: 1 Pages: 11-16
  • DOI: 10.1016/j.pdj.2018.10.004
  • Peer Reviewed
• [Presentation] 齲蝕感受性に関わる染色体領域の限定 (2018)
  • Author(s): 渡邉 千尋, 井上 雄温, 折野 大輔, 遠藤 智佳, 清水 邦彦, 清水 武彦, 三浦 保紀
  • Organizer: 第56回日本小児歯科学会大会
• [Presentation] Establishment of Congenic Mice to clarify Host Susceptibility to Caries (2017)
  • Author(s): C. Watanabe, K. Shimizu, D. Orino, T. Shimizu
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: San Francisco, Calif, USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] マウス齲蝕感受性に関与する遺伝要因の探索 (2017)
  • Author(s): 渡邉千尋，折野大輔，清水邦彦，清水武彦
  • Organizer: 第55回日本小児歯科学会大会