| Project/Area Number |
16K11838
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Research Collaborator |
Nakazawa Koji
Sano Kotaro
Moritani Yuki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | スフェロイド / 歯根膜幹細胞 / 歯周組織再生 / 歯根膜細胞 / 血管内皮細胞 |
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| Outline of Final Research Achievements |
We investigated the characteristics and potential of periodontal tissue regeneration in co-cultured spheroids of hPDLMSCs and HUVECs in vitro and in vivo. The expression levels of stemness markers, vascular endothelial growth factor (VEGF), and osteogenesis-related genes were up-regulated in co-cultured spheroids, compared with monolayer and spheroid cultures of hPDLMSCs. The nodule formation was increased in co-cultured spheroids, compared with monolayer and spheroid-cultured hPDLMSCs. Periodontal tissue defects were prepared in the maxillary first molars of rats and subjected to transplantation assay. Treatment with co-cultured spheroids enhanced new cementum formation after 4 or 8 weeks of transplantation, although there was no significant difference in the new bone formation between co-cultured spheroids and hPDLMSC spheroids. These data suggest that co-cultured spheroids enhance the periodontal tissue regeneration.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト歯根膜幹細胞（hPDLMSC）とヒト臍帯静脈内皮細胞（HUVEC）から作製した共培養スフェロイドはすでに再生能を有すると報告されている歯根膜細胞に比較して、歯周組織欠損部において、歯槽骨のみならず、歯根膜・セメント質といった組織に関しても、有意に高い再生能力を示した。hPDLMSC、HUVECの細胞の組み合わせ、並びにスフェロイドという培養形態は組織再生を目的とした細胞移植において、有効なツールとなりえる可能性が示唆された。
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