| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Rodent mandibular incisors have a unique epithelial tissue stem cell structure ‘apical bud’ that allows teeth to grow throughout the lifetime of the rodent. We had succeeded in making an animal experimental model that enables replacing the living wild type mise apical buds with GFP-positive mise ones. The tissue showed anatomically normal structure and some of the GFP-positive cells were observed as cementblast-like cells inside cementum. The experimental protocol has further tuned up as isolating pure apical bud cells from GFP-positive mouse, dispersing cells, and then injecting them to the periodontal ligament space of the wild type mouse. The injected cells survived in the periodontal ligament space and some were observed in cementum. These observations could be important findings that leads to understanding of tooth development and tissue regeneration.
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