Effect of low concentration fluoride in senescence of mesenchymal stem cells

• Project/Area Number: 16K11898
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Nihon University
• Principal Investigator: NASU Ikuo 日本大学, 松戸歯学部, 客員教授 (80112952)
• Co-Investigator(Kenkyū-buntansha): 有川 量崇 日本大学, 松戸歯学部, 教授 (50318325) ; Bhawal Ujjal 日本大学, 松戸歯学部, 助教 (50433339) ; 田口 千恵子 日本大学, 松戸歯学部, 助手(専任扱) (80434091)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 老化 / 間葉系幹細胞 / 老化促進モデルマウス / 大腿骨 / DNAマイクロアレイ / microRNAマイクロアレイ / 低濃度フッ化物 / フッ化物

Outline of Final Research Achievements

We evaluated the influence of microRNAs and their target genes on bone tissue to elucidate physiological function of aging in term of bone metabolism and autoimmunity. Total RNA was isolated from femoral tissue using 3-month-old and 15-month-old SAM mice of the aging model. Their gene expression and miRNA expression findings were analyzed using DNA microarrays and miRNA arrays in combination with GeneSpring and Ingenuity Pathways Analysis. Gene ontology (GO) analysis revealed that it incorporates critical transcription-related processes and intracellular signaling of genes targeted to miRNA. Further analysis by RT-qPCR indicates that aging regulatory transcription factors are involved in the regulation of miR-223-3p, miR-744-5p, miR-3103-5p, miR-4723-5p and miR-6825-5p. Comprehensive analysis of mRNAs and miRNAs in which numerous combinations were identified suggested that aberrant expression of miRNAs is important in bone aging.

Academic Significance and Societal Importance of the Research Achievements

今回用いるSAMP6系は、若齢期の最大骨密度が低いために加齢に伴い早期に骨粗鬆症に至りやすい老人性骨粗鬆症モデル動物である。当該研究において老化細胞マスター転写因子が制御している標的遺伝子による老化細胞の制御機構を明らかにするため、SAMP6マウスを用いてゲノムワイドにスクリーニングを展開し老化細胞制御遺伝子を同定、骨組織への影響を明らかにすることで、老化幹細胞の生理的機能の解明のみならず、異常活性および機能不全で誘発される代謝性、自己免疫性骨疾患のメカニズム解明し、再生医療の発展に役立つものと考えられる。

Research Products

• [Journal Article] 高齢者の口腔環境に対する中鎖脂肪酸とビタミンＤを含有するオイルの効果の検討 (2018)
  • Author(s): 有川量崇，谷野 弦，田口千恵子，竹内麗理，小林良喜，内山敏一， Ujial K.Bhawal，那須郁夫
  • Journal Title: 日本口腔ケア学会雑誌 Volume: 12(2) Pages: 11-16
  • NAID: 40021714626
  • Peer Reviewed
• [Journal Article] 高齢者の口腔環境に対する中鎖脂肪酸とビタミンＤを含有するオイルの効果の検討 (2017)
  • Author(s): 有川量崇，谷野 弦，田口千恵子，竹内麗理，小林良喜，内山敏一， Ujial K.Bhawal，那須郁夫
  • Journal Title: 日本口腔ケア学会雑誌 Volume: 52/ 3 Pages: 130-141
  • NAID: 40021714626
  • Peer Reviewed
• [Journal Article] Erosion by an Acidic Soft Drink of Human Molar Teeth Assessed by X-Ray Diffraction Analysis (2017)
  • Author(s): Gotouda Hiroya, Nasu Ikuo, Kono Tetsuro, Ootani Yukari, Kanno Takeshi, Tamamura Ryo, Kuwada-Kusunose Takao, Suzuki Kunihiro, Hirayama Tomohiko, Hirayama Tatsuya, Sakae Toshiro, Okada Hiroyuki
  • Journal Title: Journal of Hard Tissue Biology Volume: 26(1) Pages: 81-85
  • NAID: 130005188420
  • Peer Reviewed
• [Journal Article] Immunohistological Study of the Major Salivary Glands in the Gray Short-Tailed Opossums(Monodelphis domestica (2017)
  • Author(s): Gotouda Hiroya, Tamamura Ryo, Kono Tetsuro, Ootani Yukari, Kanno Takeshi, Kuwada-Kusunose Takao, Suzuki Kunihiro, Sakae Toshiro, Okada Hiroyuki, Nasu Ikuo
  • Journal Title: Journal of Hard Tissue Biology Volume: 26(1) Pages: 75-80
  • NAID: 130005188395
  • Peer Reviewed
• [Journal Article] 歯科診療室内の浮遊微粒子群が細胞に与える影響 (2017)
  • Author(s): 田口千恵子、小林良喜、加藤志奈、有川量崇、内山敏一、那須郁夫
  • Journal Title: 日大口腔科学 Volume: 43(1) Pages: 35-40
  • Peer Reviewed
• [Journal Article] 日本人永久歯歯数のAge-Period-Cohort分析 歯科疾患実態調査による (2016)
  • Author(s): 那須 郁夫、中村 隆
  • Journal Title: 老年歯科医学 Volume: 31(1) Pages: 39-50
  • NAID: 130005165749
  • Peer Reviewed
• [Journal Article] An Examination and Evaluation of Question Preparation for Computer-based Testing in Dental Education (2016)
  • Author(s): Gotouda Hiroya, Nishimura Hitoshi, Kageyama Makiko, Endo Hiroyasu, Wakami Masanobu, Tamamura Ryo, Okada Hiroyuki, Shimizu Takehiko, Hiratsuka Koichi, Kaneda Takashi, Hirayama Satoshi, Oomine Hirotaka, Matsushima Kiyoshi, Kawai Yasuhiko, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 15(2) Pages: 46-53
  • Peer Reviewed
• [Journal Article] Correlation between Assignments for Professionalism of the Post-graduate Clinical Competency Evaluation and the Pre-graduate Objective Structured Clinical Achievement Test in Dental Education (2016)
  • Author(s): Gotouda Hiroya, Ito Takanori, Okamoto Yasuhiro, Uchida Takashi, Taguchi Chieko, Shimosaka Michiharu, Fuchigami Mana, Fukatsu Akira, Matsune Kensuke, Kono Yoshiharu, Matsushima Kiyoshi, Komiya Masamichi, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 15(2) Pages: 40-45
  • NAID: 130005243479
  • Peer Reviewed
• [Journal Article] A Study on the Occupational Stress of Trainee Dentists in Post-graduate Dental Education (2016)
  • Author(s): Gotouda Hiroya, Ito Takanori, Okamoto Yasuhiro, Uchida Takashi, Taguchi Chieko, Shimosaka Michiharu, Fuchigami Mana, Fukatsu Akira, Matsune Kensuke, Kono Yoshiharu, Sakamaki Tatsuo, Matsushima Kiyoshi, Komiya Masamichi, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 15(2) Pages: 33-39
  • NAID: 130005243476
  • Peer Reviewed
• [Journal Article] An Examination of the Post-graduate Dental Clinical Competency Evaluation at the End of Clinical Training for Professionalism in Dental Education (2016)
  • Author(s): Gotouda Hiroya, Ito Takanori, Okamoto Yasuhiro, Uchida Takashi, Taguchi Chieko, Shimosaka Michiharu, Fuchigami Mana, Fukatsu Akira, Matsune Kensuke, Kono Yoshiharu, Matsushima Kiyoshi, Komiya Masamichi, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 15(2) Pages: 29-32
  • NAID: 130005243477
  • Peer Reviewed
• [Journal Article] Effectiveness of Computer-Based Testing Workshops in Dental Education (2016)
  • Author(s): Gotouda Hiroya, Nishimura Hitoshi, Saito Masanori, Kimoto Suguru, Endo Hiroyasu, Wakami Masanobu, Tamamura Ryo, Okada Hiroyuki, Shimizu Takehiko, Hiratsuka Koichi, Kaneda Takashi, Hirayama Satoshi, Oomine Hirotaka, Matsushima Kiyoshi, Kawai Yasuhiko, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 15(1) Pages: 17-21
  • Peer Reviewed
• [Presentation] Insights into the mechanisms of low-level fluoride in preventing periodontal disease: current status and future expectations (2018)
  • Author(s): Ujjal K. Bhawal, Yu Fujita, Ikuo Nasu, Hirohisa Arakawa
  • Organizer: 13th International Conference of the Asian Academy of Preventive Dentistry
  • Int'l Joint Research
• [Presentation] Role of stromal cell-derived factor 1 alpha and CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation (2018)
  • Author(s): Fengzhu Zhang, Ujjal K. Bhawal, Chieko Taguchi, Kazumune Arikawa, Ikuo Nasu, Hirohisa Arakawa, Koh Shibutani
  • Organizer: 第67回日本口腔衛生学会・総会
• [Presentation] Micromolar level of sodium fluoride promote osteoblast differentiation through Runx2 signaling (2017)
  • Author(s): Bhawal UK,Arikawa K,Taguchi C,Uchiyamma T,Hiratsuka K,Arakawa H,Nasu I
  • Organizer: 第66回日本口腔衛生学会・総会
• [Presentation] The effects of low-power laser irradiation in submandibullar glands of diabetes-induced rats (2017)
  • Author(s): Bhawal UK,Uchiyamma T,Arikawa K,Oka S,Hiratsuka K,,Hiratsuka K,Arakawa H,Nasu I,Shibutani K
  • Organizer: IUPS38ht World Congress
• [Presentation] Differentiated embryo chondrocyte 1 (DEC1) is a novel negative regulator of hepatic fibroblast growth factor 21 (FGF21) in aging mice (2016)
  • Author(s): Bhawal UK, Arikawa K, Taguchi C, Nakayama R, Nasu I, Arakawa H, Hiratsuka K
  • Organizer: 第65回日本口腔衛生学会・総会; The 12th International Conference of Asian Academy of Preventive Dentistry : AAPD
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] MicroRNA expression profiling and inhibition of inflammation of Korean red ginseng extract in rats on kanamycin-induced hearing loss (2016)
  • Author(s): Bhawal UK, Arikawa K, Taguchi C, Nakayama R, Nasu I, Arakawa H, Hiratsuka K
  • Organizer: 第65回日本口腔衛生学会・総会; The 12th International Conference of Asian Academy of Preventive Dentistry : AAPD
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] DNA microarray and MicroRNA analysis in low-level laser therapy (LLLT)-treated diabetic rat salivary glands (2016)
  • Author(s): Bhawal UK, Arikawa K, Taguchi C, Nakayama R, Nasu I, Arakawa H, Hiratsuka K
  • Organizer: 第65回日本口腔衛生学会・総会; The 12th International Conference of Asian Academy of Preventive Dentistry : AAPD
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] 口内炎モデルラットによる水素水の治療および予防効果の検討 (2016)
  • Author(s): 竹内 麗理, 有川 量崇, 田口 千恵子, 中山 竜司, 平塚 浩一, 那須 郁夫
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27
• [Presentation] マウスにおける分泌型IgA抗体産生の概日リズム (2016)
  • Author(s): 田口 千恵子, 小林 良喜, 有川 量崇, 中山 竜司, 落合 智子, 那須 郁夫
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27
• [Presentation] う蝕リスク診断におけるハイリスク者選別の評価法の比較検討 (2016)
  • Author(s): 後藤田 宏也, 田口 千恵子, 生田 明敏, 桑原 紀子, 松根 健介, 平塚 浩一, 河野 善治, 松村 淳, 那須 郁夫
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: 東京医科歯科大学 M&Dタワー 鈴木章夫記念講堂、東京、文京区
  • Year and Date: 2016-05-27