Design of cross-talking amplification circuit with orthogonal nucleic acid
| Project/Area Number |
16K12522
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 直交性 / DNA / RNA / 論理回路 / 人工核酸 / 蛍光増幅 / クロストーク / 増幅回路 / RNA検出 / PNA / ＤＮＡ |
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| Outline of Final Research Achievements |
Orthogonality is a key concept that has been broadly interpreted to mean that components or elements do not affect each other or behave independently. Previously, we have developed three artificial nucleic acids, D-aTNA, L-aTNA, SNA, composed of acyclic serinol derivatives as scaffolds. All of them can form remarkably stable homo-duplexes. While D-aTNA cross-pairs neither natural DNA (D-DNA) nor RNA (D-RNA), SNA with achiral scaffold can cross-pair with D-, L-aTNA, D-DNA, and D-RNA. In the present study, we designed a logic gate with D-aTNA that is orthogonal to D-RNA, which can be activated by D-RNA input via SNA as an interface: A seesaw gate with D-aTNA and interface SNA targeting miR21 was designed. Fluorescence could successfully be amplified by D-RNA (miR21) input that triggered D-aTNA seesaw gate via SNA.
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Report
(3 results)
Research Products
(18 results)
