| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The DNA damage tolerance pathways as the post-replication repair are regulated by ubiquitination of proliferating cell nuclear antigen (PCNA). Since mono- and poly-ubiquitination of PCNA stimulates the error-prone pathway, translesion DNA synthesis (TLS), and the error-free, in principle, pathway, template switch (TS), respectively. However, in humans, the regulatory mechanism is obscure because poly-ubiquitinated PCNA is only slightly detectable. In this study, we identified deubiquitinases for ubiquitinated PCNA and analyzed their functions in the damage tolerance pathways.
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