• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Multiple mechanisms of post-replication repair pathway choice by deubiquitinases for ubiquitinated PCNA

Research Project

Project/Area Number 16K12594
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionNagoya University

Principal Investigator

MASUDA Yuji  名古屋大学, 医学系研究科(環医), 准教授 (30273866)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords損傷トレランス / DNA修復 / 修復
Outline of Final Research Achievements

The DNA damage tolerance pathways as the post-replication repair are regulated by ubiquitination of proliferating cell nuclear antigen (PCNA). Since mono- and poly-ubiquitination of PCNA stimulates the error-prone pathway, translesion DNA synthesis (TLS), and the error-free, in principle, pathway, template switch (TS), respectively. However, in humans, the regulatory mechanism is obscure because poly-ubiquitinated PCNA is only slightly detectable. In this study, we identified deubiquitinases for ubiquitinated PCNA and analyzed their functions in the damage tolerance pathways.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Remarks (4 results)

  • [Journal Article] Overexpression of Rev1 promotes the development of carcinogen-induced intestinal adenomas via accumulation of point mutation and suppression of apoptosis proportionally to the Rev1 expression level.2017

    • Author(s)
      Sasatani M, Xi Y, Kajimura J, Kawamura T, Piao J, Masuda Y, Honda H, Kubo K, Mikamoto T, Watanabe H, Xu Y, Kawai H, Shimura T, Noda A, Hamasaki K, Kusunoki Y, Zaharieva EK, Kamiya K.
    • Journal Title

      Carcinogenesis.

      Volume: 38(5) Issue: 5 Pages: 570-578

    • DOI

      10.1093/carcin/bgw208

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] USP7はPCNAのユビキチン化を制御して酸化的DNA損傷による突然変異を抑制する2017

    • Author(s)
      益谷央豪, 柏葉脩一郎, 金尾梨絵, 楠本理加, 花岡文雄, 増田雄司
    • Organizer
      第34回染色体ワークショップ/第15回核ダイナミクス研究会
    • Place of Presentation
      木更津
    • Year and Date
      2017-01-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 紫外線損傷乗り越えDNA合成におけるDNAポリメラーゼηの制御機構2017

    • Author(s)
      増田雄司, 金尾梨絵, 益谷央豪
    • Organizer
      日本環境変異原学会第46回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ユビキチン化PCNAの脱ユビキチン化酵素として機能するヒトUSP7の作用機序2017

    • Author(s)
      増田雄司, 益谷央豪
    • Organizer
      日本放射線影響学会第60回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノムの安定化と不安定化をもたらす損傷乗り越えDNA複製の制御機構の解析2017

    • Author(s)
      益谷央豪, 金尾梨絵, 松尾(楠本)理加, 増田雄司
    • Organizer
      ワークショップ「加齢関連疾患の発生と治療に関わるDNA損傷と細胞老化」ConBio2017/第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] PCNAの翻訳後修飾が制御するDNA損傷トレランスの解析2016

    • Author(s)
      金尾梨絵, 増田雄司, 益谷央豪
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Suppression of PCNA ubiquitination and oxidative stress-induced mutagenesis by USP72016

    • Author(s)
      Kanao R, Kashiwaba S, Masuda Y, Kusumoto-Matsuo R, Hanaoka F, Masutani C.
    • Organizer
      The 10th 3R (Replication, Recombination and Repair) symposium
    • Place of Presentation
      Matsue
    • Year and Date
      2016-11-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] PCNAの翻訳後修飾の可逆的な制御による突然変異抑制機構の解析2016

    • Author(s)
      益谷央豪, 金尾梨絵, 柏葉脩一郎, 松尾(楠本)理加, 増田雄司
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      広島
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Mechanism of multiple lysine 48-linked ubiquitin chain synthesis by E6AP.2016

    • Author(s)
      Masuda Y, Saeki Y, Yanaka S, Kawai H, Kukimoto I, Kato K, Tanaka K, Masutani C.
    • Organizer
      FASEB Science Research Conference, UBIQUITIN & CELLULAR REGULATION
    • Place of Presentation
      Montana, U.S.A.
    • Year and Date
      2016-06-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] USP7 suppresses H2O2-induced mutagenesis by regulating PCNA ubiquitination in human cells.2016

    • Author(s)
      Masutani C, Kashiwaba S, Kanao R, Kusumoto-Matsuo R, Hanaoka F, Masuda Y
    • Organizer
      Gordon Research Conference, Mutagenesis
    • Place of Presentation
      Girona, Spain.
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 名古屋大学 環境医学研究所

    • URL

      http://www.riem.nagoya-u.ac.jp

    • Related Report
      2017 Annual Research Report
  • [Remarks] 名古屋大学 環境医学研究所 ゲノム動態制御分野

    • URL

      http://www.riem.nagoya-u.ac.jp/4/genome/home.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 名古屋大学環境医学研究所

    • URL

      http://www.riem.nagoya-u.ac.jp

    • Related Report
      2016 Research-status Report
  • [Remarks] 名古屋大学環境医学研究所ゲノム動態制御分野

    • URL

      http://www.riem.nagoya-u.ac.jp/4/genome/home.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi