| Project/Area Number |
16K12607
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental impact assessment
|
|---|
| Research Institution | Ibaraki University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 酸化損傷塩基 / 酸化ラジカル反応 / 自然突然変異 / 酸化ラジカル / DNA修復 |
|---|
| Outline of Final Research Achievements |
One of the most lesions in DNA caused by reactive oxygen species (ROS) is the oxidized base 7,8-dihydro-8-oxoguanine (8-oxoG). The product of three of the Escherichia coli mut genes, mutM, mutY, and mutT, are devoted exclusively to preventing mutations due to 8-oxoG and are important components of the defense against this type of oxidative damage. However, other bacteria for DNA repair systems to prevent 8-oxoG such as MutT are unclear. Here, we used some strains, such as an Bacillus subtilis, Lactobacillus reuteri, Pseudoalteromonas sp from deep sea, Synechocystis sp. PCC6803, and E. coli as a control. We measured the amount of 8-oxoG in chromosomal DNA. Also, cells highly accumulated 8-oxoG in DNA at low temperature than at appropriate temperature in all of bacteria. These results indicated that accumulation of 8-oxoG by ROS is highly active in exponential growth and at low temperature and prevention of DNA mutation by 8-oxoG are due to MutY and MutM rather than MutT.
|
| Academic Significance and Societal Importance of the Research Achievements |
これまで、生物進化の原動力は、ウィルス、トランスポゾン、プラスミド等による遺伝子の水平伝搬、すなわち外的要因を中心に研究されてきた。本研究は、細胞内酸化ラジカル反応により生成する酸化損傷塩基8OHdGを細胞内変異原、すなわち内的要因として位置付け、8-OHdGを生物進化の新たな指標として、そのポテンシャルを評価する点に意義がある。
|
