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Exploration and isolation of nosZ clade II type N2O-reducing bacteria by a novel enrichment device

Research Project

Project/Area Number 16K12616
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Terada Akihiko  東京農工大学, 工学(系)研究科(研究院), 准教授 (30434327)

Co-Investigator(Renkei-kenkyūsha) HORI Tomoyuki  国立研究開発法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (20509533)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords亜酸化窒素(N2O) / N2O還元細菌 / nosZ clade IIタイプ / 集積培養装置 / アンプリコンシーケンス / 呼吸活性 / 動力学的解析 / 亜酸化窒素 / 亜酸化窒素還元細菌 / nosZ clade II / 生物学的窒素除去 / アンプリコンシーケンス解析 / 基質親和性
Outline of Final Research Achievements

To mitigate emission of N2O, known as a highly-potent greenhouse and ozone-depleting gas, this study aimed at enriching and isolating nosZ clade II type N2O-reducing bacteria as promising N2O consumers with high efficiency. By developing a novel enrichment technology, Azospira sp., affiliated with nosZ clade II type, was successfully isolated. One of the strains in the bacterium, Azospira sp. strain I09, showed N2O-reducing activity in the presence of oxygen. Immobilization of Azospira sp. strain I13 into alginate gel allowed consumption of N2O even under microaerophilic conditions. To explore non-denitrifying N2O-reducing bacteria, an anammox reactor was subjected to the microbial community analysis. The analysis underpinned that the phylum Chloroflexi, detected with a relatively high abundance following anammox bacteria, was a potential candidate as non-denitrifying N2O-reducing bacteria.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Biokinetic characterization and activities of N2O-reducing bacteria in response to various oxygen level2018

    • Author(s)
      Toshikazu Suenaga、S. Riya、M. Hosomi、A. Terada
    • Journal Title

      Frontiers in Microbiology

      Volume: 9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Counter-diffusion biofilms have lower N 2 O emissions than co-diffusion biofilms during simultaneous nitrification and denitrification: Insights from depth-profile analysis2017

    • Author(s)
      Kinh Co Thi、Suenaga Toshikazu、Hori Tomoyuki、Riya Shohei、Hosomi Masaaki、Smets Barth F.、Terada Akihiko
    • Journal Title

      Water Research

      Volume: 124 Pages: 363-371

    • DOI

      10.1016/j.watres.2017.07.058

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 酸素の存在が亜酸化窒素の還元活性に及ぼす影響:単離菌株による呼吸活性の動力学的評価2017

    • Author(s)
      末永俊和、堀知行、利谷翔平、細見正明、寺田昭彦
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 亜酸化窒素を還元する脱窒細菌の動力学的解析:酸素暴露の影響2017

    • Author(s)
      末永俊和、中川洋祐、堀知行、利谷翔平、細見正明、寺田昭彦
    • Organizer
      化学工学会 第82年会
    • Place of Presentation
      芝浦工業大学(東京)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Anammoxリアクターに潜在するN2Oを巡るメタボリズム2017

    • Author(s)
      末永俊和、佐光俊樹、堀知行、細見正明、寺田昭彦
    • Organizer
      第20 回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] バイオフィルムに関する最新の研究動向:形成抑制から利用技術まで2017

    • Author(s)
      寺田昭彦
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Nitrous oxide emission from cost-effective nitrogen removal systems: Mechanisms and mitigation strategies2017

    • Author(s)
      A. Terada
    • Organizer
      2017 International Conference on Environmental Pollution Control
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 亜酸化窒素還元に寄与する脱窒細菌の亜酸化窒素と酸素を巡るダイナミクスの動力学的評価2016

    • Author(s)
      末永俊和、堀知行、利谷翔平、細見正明、寺田昭彦
    • Organizer
      日本微生物生態学会第 31 回横須賀大会
    • Place of Presentation
      横須賀市文化会館(神奈川)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Exploring N2O-reducing bacteria in anammox-based systems towards mitigation of N2O emission2016

    • Author(s)
      Toshikazu Suenaga, Co Thi Kinh, Daisuke Ikeda, Koki Maruo, Kang Song, Tomoyuki Hori, Masaaki Hosomi, Akihiko Terada
    • Organizer
      Anammox Workshop at MEWE-Biofilm
    • Place of Presentation
      コペンハーゲン(デンマーク)
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Biokinetic characterization of nitrous oxide reduction by denitrifying bacteria possessing typical and atypical nosZ genes2016

    • Author(s)
      Toshikazu Suenaga, Yosuke Nakagawa, Tomoyuki Hori, Masaaki Hosomi, Akihiko Terada
    • Organizer
      ISME 16
    • Place of Presentation
      モントリオール(カナダ)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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