Exploration and isolation of nosZ clade II type N2O-reducing bacteria by a novel enrichment device
| Project/Area Number |
16K12616
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental engineering and reduction of environmental burden
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Terada Akihiko 東京農工大学, 工学(系)研究科(研究院), 准教授 (30434327)
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| Co-Investigator(Renkei-kenkyūsha) |
HORI Tomoyuki 国立研究開発法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (20509533)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 亜酸化窒素（N2O） / N2O還元細菌 / nosZ clade IIタイプ / 集積培養装置 / アンプリコンシーケンス / 呼吸活性 / 動力学的解析 / 亜酸化窒素 / 亜酸化窒素還元細菌 / nosZ clade II / 生物学的窒素除去 / アンプリコンシーケンス解析 / 基質親和性 |
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| Outline of Final Research Achievements |
To mitigate emission of N2O, known as a highly-potent greenhouse and ozone-depleting gas, this study aimed at enriching and isolating nosZ clade II type N2O-reducing bacteria as promising N2O consumers with high efficiency. By developing a novel enrichment technology, Azospira sp., affiliated with nosZ clade II type, was successfully isolated. One of the strains in the bacterium, Azospira sp. strain I09, showed N2O-reducing activity in the presence of oxygen. Immobilization of Azospira sp. strain I13 into alginate gel allowed consumption of N2O even under microaerophilic conditions. To explore non-denitrifying N2O-reducing bacteria, an anammox reactor was subjected to the microbial community analysis. The analysis underpinned that the phylum Chloroflexi, detected with a relatively high abundance following anammox bacteria, was a potential candidate as non-denitrifying N2O-reducing bacteria.
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Report
(3 results)
Research Products
(12 results)
