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Establishment of in vivo imaging methods for visualization of beige adipose cells and functional identification of anti-obesity components.

Research Project

Project/Area Number 16K12728
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionWakayama Medical University

Principal Investigator

IHARA HAYATO  和歌山県立医科大学, 共同利用施設, 講師 (00223298)

Co-Investigator(Kenkyū-buntansha) 向阪 彰  和歌山県立医科大学, 医学部, 准教授 (00458051)
竹島 健  和歌山県立医科大学, 医学部, 助教 (40647517)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生体イメージング / 抗肥満成分 / UCP-1 / ベージュ脂肪細胞 / UCP-1遺伝子 / サンショウ成分 / Thermo Mouse / ベージュ化脂肪細胞 / 生体イメージング法 / 抗肥満機能成分 / 栄養学 / 機能性食品成分 / 抗肥満
Outline of Final Research Achievements

We have studied the imaging methods for visualization of beige adipose cells using in vitro, in vivo systems. Among these studies, we thought this in vivo system using UCP-1/Luciferase reporter transgenic mice could be useful to identify anti-obesity compounds from natural foods. After oral administration of Sansho compounds (3% in diet) to the transgenic reporter mice, we used the IVIS-XR Imaging System to monitor luciferase activity in these transgenic mice. In abdominal tissue, we first detected luciferase signal in 4 weeks after administration of Sansho compounds and luciferase signal was increased every week. In some cases, we ectopically detected luciferase signal in testis. These observations indicate that this in vivo system would be useful as a screening tool. We need further investigation to assess tight correlation between luciferase activity and endogenous UCP-1 protein expression level.

Academic Significance and Societal Importance of the Research Achievements

今や国民の6人に1人が、メタボリック症候群もしくはその予備軍と考えられており、肥満症の予防・治療方法の確立は喫緊の課題である。蓄積エネルギー消費亢進機構に基づく抗肥満薬は今の所ない。我々は、これまでサンショウにはベージュ化誘導を伴う抗肥満効果があることを示してきたが、有効成分が明らかではないため、熱産生タンパク遺伝子発現を指標に、生体イメージング方による抗肥満効果を示す成分を同定する方法を検証した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Impact of heart-specific disruption of the circadian clock on systemic glucose metabolism in mice2017

    • Author(s)
      Nakao T, Kohsaka A, Otsuka T, Thein ZL, Le HT, Waki H, Gouraud SS, Ihara H, Nakanishi M, Sato F, Muragaki Y, Maeda M.
    • Journal Title

      Chronobiol Int

      Volume: in press Issue: 4 Pages: 499-510

    • DOI

      10.1080/07420528.2017.1415922

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ANOMALOUS CHANGES IN ATMOSPHERIC RADON CONCENTRATION BEFORE AND AFTER THE 2011 NORTHERN WAKAYAMA EARTHQUAKE (MJ 5.5)2016

    • Author(s)
      Goto,M, Yasuoka,Y*, Nagahama, H, Muto, J., Omori,Y., Ihara,H., Mukai, T
    • Journal Title

      Radiation Protection Dosimetry

      Volume: - Pages: 1-7

    • DOI

      10.1093/rpd/ncw142

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 和歌山県産サンショウの抗肥満効果について2017

    • Author(s)
      井原勇人
    • Organizer
      メディカルジャパン2017  研究成果起業家促進セミナー 招待講演
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 和歌山県産サンショウの抗肥満効果の解析2017

    • Author(s)
      井原勇人、竹島健、山下雅礼、門脇昭夫、大西沙代、岸田邦博、我藤伸樹、赤水尚史
    • Organizer
      第71回 日本栄養食糧学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Anti-obesity effects of Japanese pepper, Sansho, in high-fat diet-induced obese mice through induction of beige adipocytes(白色脂肪細胞のベージュ化を伴うサンショウの抗肥満効果の解析)2017

    • Author(s)
      井原勇人、竹島健、山下雅礼、門脇昭夫、大西沙代、岸田邦博、我藤伸樹、赤水尚史
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] 白色脂肪細胞のベージュ化を伴うサンショウの抗肥満効果の解析2017

    • Author(s)
      井原勇人、竹島健、山下雅礼、門脇昭夫、大西沙代、岸田邦博、我藤伸樹、赤水尚史
    • Organizer
      第15回 日本機能性食品医用学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Heart-specific disruption of Bmal 1 leads to hepatic insulin resistance in mice.2017

    • Author(s)
      Nakao, T., Kohsaka, A., Stuka, T., Le, H.T., Thein, Z.L., Sato, F., Ihara, H., Murasaki, Y., Maeda, M.
    • Organizer
      Experimental Biology 2017 (EB2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 心臓特異的な時計遺伝子Bmal 1の欠損は肝臓のインスリン抵抗性を惹起する2017

    • Author(s)
      中尾友美、向阪彰、レティ ファオ、大塚剛司、テン ソーレン、佐藤冬樹、井原勇人、前田正信
    • Organizer
      第90回 日本内分泌学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Comparative transcriptome analysis between Bmal1 and Rev-erbα deficient hearts reveals the transcriptional network linking clock and heart function.2016

    • Author(s)
      中尾友美、向阪 彰、レ ティ フォエ、大塚剛司、テン ソウ レン、佐藤冬樹、井原勇人、村垣泰光、前田正信
    • Organizer
      第23回 日本時間生物学会学術大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
  • [Presentation] 時計遺伝子欠損マウスのトランスクリプトーム解析による慢性心不全の原因遺伝子の探索2016

    • Author(s)
      中尾友美、向阪 彰、レ ティ フォエ、大塚剛司、テン ソウ レン、佐藤冬樹、井原勇人、前田正信
    • Organizer
      第109回 近畿生理学談話会
    • Place of Presentation
      大阪市立大学
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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