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Studies on detection of foodborne pathogenic bacteria in VBNC state and resuscitation condition to culturable state

Research Project

Project/Area Number 16K12737
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionHiroshima University

Principal Investigator

Shimamoto Tadashi  広島大学, 生物圏科学研究科, 教授 (90187443)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsVBNC / コレラ菌 / Vibrio cholerae / 腸炎ビブリオ / Vibrio parahaemolyticus / ビブリオ / viable but nonculturable / Vibrio / 食品 / 細菌 / 食中毒
Outline of Final Research Achievements

Bacteria of viable but nonculturable (VBNC) conditions cannot be detected by general culture methods, but it became apparent that under certain conditions, it is possible to resuscitate to the culturable state. In this study, we investigated the effect of salt concentration on transition of Vibrio cholerae O139 and V. parahaemolyticus to VBNC state and resuscitation to a culturable state.
As the results, it became clear that salt concentration is one of the important factors for transition to VBNC state and resuscitation to the culturable state.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2018 2017 2016

All Presentation (3 results)

  • [Presentation] コレラ菌のVBNC状態への移行に対する塩濃度の影響2018

    • Author(s)
      島本 敏,成谷 宏文,島本 整
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ビブリオ属細菌がVBNC状態に移行する条件の検討2017

    • Author(s)
      島本 敏,岡田 佳奈,成谷 宏文,島本 整
    • Organizer
      第51回ビブリオシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 難培養状態のコレラ菌が培養可能状態に復帰する条件の検討2016

    • Author(s)
      島本 整
    • Organizer
      第50回腸炎ビブリオシンポジウム
    • Place of Presentation
      大阪大学微生物鋲研究所
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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