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Excessive ingestion of sugar causes the abnomality of endoplasmic reticulum, resulting in providing for NASH

Research Project

Project/Area Number 16K12742
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionKanazawa Medical University

Principal Investigator

UEDA Tadashi  金沢医科大学, 総合医学研究所, 助教 (80151794)

Research Collaborator TAKEUCHI Masayoshi  金沢医科大学, 総合医学研究所, 教授 (20154982)
TAKATA Takanobu  金沢医科大学, 総合医学研究所, 助教 (20515308)
Project Period (FY) 2016-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords終末糖化タンパク / グリセルアルデヒド / 初代培養心筋細胞 / 初代培養肝細胞 / 小胞体ストレス / NASH / オートファジー / 電子顕微鏡 / 食習慣 / 終末糖化産物
Outline of Final Research Achievements

It is suggested that glyceraldehyde-derived advanced glycation products (GA-AGEs) play a key role in various diseases. We examined the effect of GA, which is a precursor of GA-AGEs, at concentrations between 0-4 mM during a period of 24 h in the primary cultured cardiomyocytes and hepatocytes. Both cultured cells were affected severely by GA, and endoplasmic reticulum (ER) was the target organelle, and thereafter mitochondria(Mt) were disrupted. Cultured hepatocytes appeared with the characteristics similar to NASH, which contained numerous lipid droplets and vacuoles. The cells which were affected by ER stress suppressed autophagy. Numerous hepatocytes in NASH model mice appeared positive against anti-TAGE antibody. Moreover, they showed ballooning cells, and ER and Mt were collapsed.
These results suggested that accumulated TAGE may cause NASH and ER plays a key role of induction into NASH.

Report

(2 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (1 results)

All 2017

All Presentation (1 results)

  • [Presentation] 初代培養心筋、肝細胞における終末糖化産物の影響2017

    • Author(s)
      上田忠司、高田尊信、竹内正義
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2018-03-22  

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