Effects of Atmospheric-pressure plasma irradiation on insulin-target cells
| Project/Area Number |
16K12863
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
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| Co-Investigator(Renkei-kenkyūsha) |
Kaneko Toshiro 東北大学, 大学院工学研究科, 教授 (30312599)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 大気圧プラズマ / 細胞 / プラズマ医療 / カルシウム / チャネル / 医工学 / 生活習慣病 / 細胞生理学 / 生理学 / 糖尿病 / GLUT4 / シグナル伝達 / プラズマ |
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| Outline of Final Research Achievements |
Recently, non-equilibrium atmospheric pressure plasma (APP) emerged to exert diverse biological responses, though the underlying mechanisms remained to be determined. This study aimed to explore precise mechanisms underlying of plasma-inducible increases in intracellular Ca2+ concentration in insulin-responsive cells, which has been recently discovered by our research group. Our results demonstrated that increases in intracellular Ca2+ are induced by continuous low-dose O2－/ ONOO－ supply, which is mediated through TRP channel activation. Our findings indicate the potential of APP for regulating insulin-responsive cell functions. Moreover, APP showed inhibitory actions on adipogenic differentiation of 3T3L1 cells. Together, these results demonstrate that APP-induced TRP channel activation is intimately involved in various cellular responses triggered by APP exposure.
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Report
(3 results)
Research Products
(48 results)
